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A novel protein with a fibrinogen-like domain involved in the innate immune response of Marsupenaeus japonicus
- Source :
- Fish & Shellfish Immunology. 32:307-315
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Fibrinogen-related proteins play important roles in innate immunity. We isolated a fibrinogen-related protein gene (MjFREP1) in kuruma shrimp Marsupenaeus japonicus. MjFREP1 encoded a protein of 270 amino acids, including a 223 amino acid fibrinogen-like domain. Quantitative real-time polymerase chain reaction analysis shows that MjFREP1 is mainly expressed in the gills and the expression is significantly upregulated by Vibrio anguillarum, Staphylococcus aureus, or white spot syndrome virus (WSSV) challenge. Recombinant MjFREP1 fibrinogen-like domain agglutinates Gram-positive bacteria Bacillus subtilis, Bacillus thuringiensis, Bacillus megaterium, and S. aureus in the presence of calcium ions. The fibrinogen-like domain of MjFREP1 binds peptidoglycans, LPS, bacteria, and the VP28 of WSSV. These results suggest that the MjFREP1 may play an important role in the shrimp immune response against different pathogens.
- Subjects :
- Vibrio anguillarum
Molecular Sequence Data
White spot syndrome
Bacillus subtilis
Aquatic Science
Microbiology
White spot syndrome virus 1
Protein structure
Immune system
Penaeidae
Animals
Environmental Chemistry
Amino Acid Sequence
Peptide sequence
Phylogeny
Bacillus megaterium
Innate immune system
Bacteria
Base Sequence
biology
Escherichia coli Proteins
Gene Expression Profiling
Phosphotransferases
fungi
Fibrinogen
Membrane Proteins
General Medicine
biology.organism_classification
Immunity, Innate
Recombinant Proteins
Protein Structure, Tertiary
Sequence Analysis
Protein Binding
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....15ef28f959aabd935fbb5933e552f8e0