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Amplification of the PPFIA1 gene region on 11q13 in oral squamous cell carcinomas (OSCC)
- Source :
- Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery. 41(8)
- Publication Year :
- 2012
-
Abstract
- Analyzing chromosomal amplifications delivers valuable information for identification of oncogenes. For carcinomas of the oral cavity only few genes have been identified in amplified regions. The aim of this study was to search genes in amplified regions as possible biomarkers and targets for novel therapies.DNA from 10 carcinomas of the floor of the oral cavity was examined using a 500K Array GeneChip (Affymetrix 6.0) to detect chromosomal losses, gains or amplifications. Suspicious alterations were validated on tissue microarrays using fluorescence in situ hybridization (FISH) with respective probes.FISH-validation on tissue arrays confirmed PPFIA1 amplifications as one of the most frequent events (32.6%). High (10-20 signals) and low (10 signals) amplification of PPFIA1 was found in 10.9% (5/46) and 21.7% (10/46) tumours, respectively. Fine mapping with overlapping FISH probes showed co-amplification of PPFIA1 and the Cyclin D1 gene which are approximately 600 kb apart from each other, likely in the same amplicon.PPFIA1 was frequently co-amplified with the Cyclin D1 gene in oral carcinomas and could present a biomarker as well as a novel target for specific gene therapy. Further studies are necessary to investigate the role of PPFIA1 in development and pathogenesis of oral carcinomas.
- Subjects :
- Male
Genetic enhancement
Cell
Biology
Cyclin D1
medicine
Biomarkers, Tumor
Humans
Gene
Laryngeal Neoplasms
In Situ Hybridization, Fluorescence
Adaptor Proteins, Signal Transducing
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
Tissue microarray
medicine.diagnostic_test
Chromosomes, Human, Pair 11
Gene Amplification
Pharyngeal Neoplasms
DNA, Neoplasm
Molecular biology
medicine.anatomical_structure
Otorhinolaryngology
Tissue Array Analysis
Gene chip analysis
Carcinoma, Squamous Cell
Biomarker (medicine)
Surgery
Female
Mouth Neoplasms
Oral Surgery
Neoplasm Grading
DNA Probes
Cell Adhesion Molecules
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 18784119
- Volume :
- 41
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery
- Accession number :
- edsair.doi.dedup.....1600e290a08f077c03ce62be13198388