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Postconditioning Ozone Alleviates Ischemia-Reperfusion Injury and Enhances Flap Endurance in Rats
- Source :
- Journal of Investigative Surgery. 33:15-24
- Publication Year :
- 2018
- Publisher :
- Informa UK Limited, 2018.
-
Abstract
- PubMed: 30339503<br />Muscle-flap transferring is a routine approach utilized in reconstructive operations; however, flap morbidity is often a source of post-operative difficulty. Ischemia-Reperfusion Injury (IRI) is an important contributor to the viability of flaps after transferring. The goal of this research was for assess the probable useful impacts of ozone on flap survival in a rat muscle-flap design. Materials and Methods: We examined the effects of postconditioning ozone administration on viability of pedicled composite flaps. Twenty-eight Wistar rats were randomized into four groups: sham-operated (S), ischemia-reperfusion (IR), sham-operated + ozone (O), IR + ozone (IR + O), respectively. The animals were sacrificed on the eighth day. In a general histological evaluation, flap tissues were examined with a light microscope, and apoptotic cells were counted. The Apoptotic Index (AI) was then calculated. Flap-tissue samples were sent for analyses of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and protein carbonyl (PCO), and blood samples were sent for analyses of Total Oxidant Score (TOS), and Total Antioxidant Capacity (TAC). Data were evaluated statistically using the Kruskal–Wallis test. Results: The histomorphometric score was remarkably greater in O (p =.002). The AI was greater in IR (p =.002). The antioxidant parameters values as regards SOD, GSH-Px, CAT, and TAC were found to be greater in O (p
- Subjects :
- Male
antioxidant
Ischemia
surgical flaps
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Rats, Wistar
Surgical Flaps
Ischemic Postconditioning
ischemia reperfusion injury
business.industry
Graft Survival
apoptosis
medicine.disease
eye diseases
Rats
Disease Models, Animal
Oxidative Stress
ozone
Reperfusion Injury
030220 oncology & carcinogenesis
Anesthesia
030211 gastroenterology & hepatology
Surgery
business
Reperfusion injury
Injections, Intraperitoneal
Subjects
Details
- ISSN :
- 15210553 and 08941939
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Surgery
- Accession number :
- edsair.doi.dedup.....164f441f9646aa343554c0d3195d57a2