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Mlh1 interacts with both Msh2 and Msh6 for recruitment during mismatch repair
- Publication Year :
- 2022
- Publisher :
- eScholarship, University of California, 2022.
-
Abstract
- Eukaryotic DNA mismatch repair (MMR) initiates through mispair recognition by the MutS homologs Msh2-Msh6 and Msh2-Msh3 and subsequent recruitment of the MutL homologs Mlh1-Pms1 (human MLH1-PMS2). In bacteria, MutL is recruited by interactions with the connector domain of one MutS subunit and the ATPase and core domains of the other MutS subunit. Analysis of the S. cerevisiae and human homologs have only identified an interaction between the Msh2 connector domain and Mlh1. Here we investigated whether a conserved Msh6 ATPase/core domain-Mlh1 interaction and an Msh2-Msh6 interaction with Pms1 also act in MMR. Mutations in MLH1 affecting interactions with both the Msh2 and Msh6 interfaces caused MMR defects, whereas equivalent pms1 mutations did not cause MMR defects. Mutant Mlh1-Pms1 complexes containing Mlh1 amino acid substitutions were defective for recruitment to mispaired DNA by Msh2-Msh6, did not support MMR in reconstituted Mlh1-Pms1-dependent MMR reactions in vitro, but were proficient in Msh2-Msh6-independent Mlh1-Pms1 endonuclease activity. These results indicate that Mlh1, the common subunit of the Mlh1-Pms1, Mlh1-Mlh2, and Mlh1-Mlh3 complexes, but not Pms1, is recruited by Msh2-Msh6 through interactions with both of its subunits.
- Subjects :
- Saccharomyces cerevisiae Proteins
Protein-protein interactions
DNA repair
Saccharomyces cerevisiae
Biochemistry
DNA Mismatch Repair
Genetics
Humans
2.1 Biological and endogenous factors
Aetiology
Molecular Biology
Mismatch Repair Endonuclease PMS2
Adenosine Triphosphatases
Msh2-Msh6
Cell Biology
DNA
Endonucleases
DNA-Binding Proteins
MutS Homolog 2 Protein
MutL Proteins
Mutagenesis
Mlh1-Pms1
Biochemistry and Cell Biology
MutL Protein Homolog 1
Mispaired base
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....166a87476c17ee868353eb6228023a4e