Additional file 1 of Dual-stimuli responsive smart nanoprobe for precise diagnosis and synergistic multi-modalities therapy of superficial squamous cell carcinoma

Additional file 1: Fig. S1. The photographs of the crystallization of FeIIITA complex and biomineralization of FeIIITA@HA nanoprobes at 0 and 24 h, for showing the solubility and stability of the crystals. Fig. S2. The histogram showing the size distribution profile of FeIIITA@HA nanoprobes. Fig. S3. Temperature variation of solutions containing FeIIITA@HA nanoprobes with the irradiation of 650 nm laser with different power densities. Fig. S4. Determination of the system time constant using linear regression of the cooling profile after irradiation of 650 nm laser. Fig. S5. The linear regression fitting of the R2 values of aqueous solutions of FeIIITA@HA nanoprobes with different Fe concentrations for extracting the transverse molar relaxivity r2. Fig. S6. The binding of FITC-labeled CD44 Ab on unblocked and blocked SCC-9 cells, together with the quantitative analysis of the fluorescence signals. Fig. S7. The binding of 5-AF-labeled HA on unblocked and blocked SCC-9 cells, together with the quantitative analysis of the fluorescence signals. Fig. S8. The integrated blue signals of the field of view of the Prussian staining of cells. Fig. S9. CD44 staining of the different regions of SCC-9 tumor slice. Fig. S10. Prussian staining of tissue slices from major organs of mice treated with FeIIITA@HA nanoprobes or PBS for showing the retention of nanoprobes. Fig. S11. H&E staining of tissue slices from major organs of mice treated with FeIIITA@HA nanoprobes or PBS. Fig. S12. Temporal evolution of relative R1 values of the liver region, spleen region, renal cortex region, and renal pelvis region of mice. Together with the calculation of the photothermal conversion efficiency.