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Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta

Authors :
Mingyu Jiang
Mingyong Ren
Jicheng Dai
Chunming Jiang
Rong Fu
Xu Liu
Yanbo Pan
Yunpeng Hao
Jiale Feng
Source :
Aging (Albany NY)
Publication Year :
2021
Publisher :
Impact Journals, LLC, 2021.

Abstract

We investigated the influence of signal transducer and activator of transcription-3 (STAT3) on the spinal cord tissue grafts of rat fetuses with spina bifida aperta. In particular, we hoped to identify whether transfection of the STAT3 overexpression plasmid increases the survival of spinal cord transplantation in order to improve therapeutic efficacy. The fetal rat model of spina bifida aperta was established using retinoic acid and treated with a microsurgical injection of bone marrow mesenchymal stem cells (BMSCs). The animals were divided into either the blank control group, negative control group or the experimental group. The optical density (OD) value of BMSCs viability was determined using the Cell Counting Kit-8 (CCK-8). The expression of STAT3, phosphorylated STAT3 (pSTAT3), neural markers and apoptosis-related factors were evaluated using real-time PCR and Western blot. The OD value in the experimental group was highest at eight hours after transplantation using CCK-8. The expression of pSTAT3, glial fibrillary acidic protein, neuron-specific enolase, neurofilament and nestin in the experimental group was significantly higher compared to the blank control group and negative control group (P

Details

ISSN :
19454589
Volume :
13
Database :
OpenAIRE
Journal :
Aging
Accession number :
edsair.doi.dedup.....167eb46030c19cbfa154e9e57223be30
Full Text :
https://doi.org/10.18632/aging.203524