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Control of late G0/G1 progression and protein modification by SmC/IGF I
Control of late G0/G1 progression and protein modification by SmC/IGF I
- Source :
- American Journal of Physiology-Cell Physiology. 253:C575-C579
- Publication Year :
- 1987
- Publisher :
- American Physiological Society, 1987.
-
Abstract
- Somatomedin C/insulin-like growth factor I (SmC/IGF I) mediates traverse of late G0/G1 in density-arrested BALB/c-3T3 cells from a distinct growth arrest point in mid-G0/G1 (the V point) to the initiation of DNA synthesis. As a prelude to future studies aimed at defining the mechanism of action of SmC/IGF I, we investigated the level (e.g., transcriptional, translational) at which SmC/IGF I modulates V to S traverse. The post-V point progression of cells arrested at the V point by amino acid starvation and released into amino acid-replenished medium containing SmC/IGF I, insulin, or platelet-poor plasma (PPP) did not require either mRNA synthesis or an increase in the overall level of protein synthesis. Although two-dimensional gel analysis of proteins prepared from SmC/IGF I-treated cells did not reveal any preferentially synthesized proteins, several SmC/IGF I-induced protein modifications, which result in an increase in isoelectric point (pI) and occur in the absence of mRNA synthesis, were evident. These findings suggest that SmC/IGF I modulates late G0/G1 progression by a posttranscriptional process that may involve protein modification.
- Subjects :
- DNA Replication
medicine.medical_specialty
Physiology
medicine.medical_treatment
Biology
Mice
Somatomedins
Internal medicine
medicine
Protein biosynthesis
Animals
Insulin
Isoelectric Point
RNA, Messenger
Amino Acids
Insulin-Like Growth Factor I
Interphase
Uridine
chemistry.chemical_classification
Mice, Inbred BALB C
DNA synthesis
Growth factor
Proteins
G0 phase
Cell Biology
Cell cycle
Somatomedin
Amino acid
Cell biology
Endocrinology
chemistry
Cell culture
Electrophoresis, Polyacrylamide Gel
Subjects
Details
- ISSN :
- 15221563 and 03636143
- Volume :
- 253
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Cell Physiology
- Accession number :
- edsair.doi.dedup.....1683b29b6d128c4355d0cd586f97c001
- Full Text :
- https://doi.org/10.1152/ajpcell.1987.253.4.c575