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Expression of Cyr61, CTGF, and WISP-1 correlates with clinical features of lung cancer
- Source :
- PLoS ONE, Vol 2, Iss 6, p e534 (2007), PLoS ONE, PloS one, vol 2, iss 6
- Publication Year :
- 2007
- Publisher :
- Public Library of Science (PLoS), 2007.
-
Abstract
- Background CCN family, comprising six members (Cyr61, CTGF, Nov, WISP-1, WISP-2, WISP-3), is involved in the stimulation of cell proliferation, migration, adhesion, angiogenesis, and tumorigenesis. Several studies have shown that expression of Cyr61, CTGF, and WISP-1 affects the tumorigenic potential of lung cancer cells in vitro. However, the correlation of expression of CCN family proteins and clinical features of lung cancer remains unknown. Methodology and Principal Findings In the present work, we quantified the mRNA levels of Cyr61, CTGF, and WISP-1 in samples from 60 primary lung cancers and their matched normal lung tissues by quantitative real-time PCR assay. Downregulation of the Cyr61 and CTGF genes and upregulation of the WISP-1 gene were found in primary lung cancers compared to the paired normal lung tissues. Immunohistochemistry analysis also disclosed a similar expression pattern of Cyr61, CTGF, and WISP-1 protein in paired lung cancer tissues. Statistical analysis revealed significant associations between expression of either Cyr61 or CTGF with tumor stage, tumor histology, metastasis, smoking, and family history at diagnosis. A significant correlation also existed between WISP-1 expression with tumor histology, and patient age. Moreover, expression levels of Cyr61 and CTGF correlated with survival of the lung-cancer patients. Conclusions Our results suggest that Cyr61, CTGF, and WISP-1 might be implicated in the development and progression of primary lung cancers, and their levels might serve as valuable prognostic markers, as well as potential targets for therapeutic intervention.
- Subjects :
- Male
Pathology
Lung Neoplasms
Angiogenesis
Messenger
lcsh:Medicine
medicine.disease_cause
Metastasis
Immunoenzyme Techniques
Carcinoma, Non-Small-Cell Lung
2.1 Biological and endogenous factors
Aetiology
Non-Small-Cell Lung
lcsh:Science
Lung
Cancer
screening and diagnosis
Multidisciplinary
Tumor
integumentary system
Reverse Transcriptase Polymerase Chain Reaction
Lung Cancer
Intracellular Signaling Peptides and Proteins
Middle Aged
Prognosis
Survival Rate
Detection
CYR61
Female
Research Article
medicine.medical_specialty
General Science & Technology
Biology
CCN Intercellular Signaling Proteins
Rare Diseases
Proto-Oncogene Proteins
medicine
Biomarkers, Tumor
Humans
RNA, Messenger
Lung cancer
Oncology/Lung Cancer
Neoplasm Staging
Cell growth
Carcinoma
lcsh:R
Connective Tissue Growth Factor
medicine.disease
4.1 Discovery and preclinical testing of markers and technologies
CTGF
Case-Control Studies
Cancer research
RNA
lcsh:Q
Carcinogenesis
Biomarkers
Cysteine-Rich Protein 61
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 2
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....169187c49e3b8100c9e02e30c6f20408