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Microliter ultrafast centrifuge platform for size-based particle and cell separation and extraction using novel omnidirectional spiral surface acoustic waves
- Source :
- Lab on a chip, vol 21, iss 5
- Publication Year :
- 2021
- Publisher :
- Royal Society of Chemistry (RSC), 2021.
-
Abstract
- Asymmetric surface acoustic waves have been shown useful in separating particles and cells in many microfluidics designs, mostly notably sessile microdroplets. However, no one has successfully extracted target particles or cells for later use from such samples. We present a novel omnidirectional spiral surface acoustic wave (OSSAW) design that exploits a new cut of lithium niobate, 152 Y-rotated, to rapidly rotate a microliter sessile drop to ∼10 g, producing efficient multi-size particle separation. We further extract the separated particles for the first time, demonstrating the ability to target specific particles, for example, platelets from mouse blood for further integrated point-of-care diagnostics. Within ∼5 s of surface acoustic wave actuation, particles with diameter of 5 μm and 1 μm can be separated into two portions with a purity of 83% and 97%, respectively. Red blood cells and platelets within mouse blood are further demonstrated to be separated with a purity of 93% and 84%, respectively. These advancements potentially provide an effective platform for whole blood separation and point-of-care diagnostics without need for micro or nanoscale fluidic enclosures.
- Subjects :
- Materials science
Microfluidics
Lithium niobate
Biomedical Engineering
Bioengineering
Cell Separation
Biochemistry
Analytical Chemistry
Mice
chemistry.chemical_compound
Engineering
Sessile drop technique
Animals
Fluidics
Centrifuge
business.industry
Surface acoustic wave
General Chemistry
Acoustic wave
Sound
chemistry
Chemical Sciences
Optoelectronics
Particle
business
Subjects
Details
- ISSN :
- 14730189 and 14730197
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Lab on a Chip
- Accession number :
- edsair.doi.dedup.....16bc79d1825f8537e0c96483b3de314c