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Autophagy machinery controls nitrogen remobilization at the whole‐plant level under both limiting and ample nitrate conditions in Arabidopsis
- Source :
- New Phytologist, New Phytologist, Wiley, 2012, 194 (3), pp.732-740. ⟨10.1111/j.1469-8137.2012.04084.x⟩, New Phytologist, Wiley, 2012, 194 (3), pp.732-740. 〈10.1111/j.1469-8137.2012.04084.x〉
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- • Processes allowing the recycling of organic nitrogen and export to young leaves and seeds are important determinants of plant yield, especially when plants are nitrate-limited. Because autophagy is induced during leaf ageing and in response to nitrogen starvation, its role in nitrogen remobilization was suspected. It was recently shown that autophagy participates in the trafficking of Rubisco-containing bodies to the vacuole. • To investigate the role of autophagy in nitrogen remobilization, several autophagy-defective (atg) Arabidopsis mutants were grown under low and high nitrate supplies and labeled with at the vegetative stage in order to determine (15) N partitioning in seeds at harvest. Because atg mutants displayed earlier and more rapid leaf senescence than wild type, we investigated whether their defects in nitrogen remobilization were related to premature leaf cell death by studying the stay-green atg5.sid2 and atg5.NahG mutants. • Results showed that nitrogen remobilization efficiency was significantly lower in all the atg mutants irrespective of biomass defects, harvest index reduction, leaf senescence phenotypes and nitrogen conditions. • We conclude that autophagy core machinery is needed for nitrogen remobilization and seed filling.
- Subjects :
- [SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences
Physiology
Mutant
Arabidopsis
NATURAL VARIATION
Plant Science
Vacuole
01 natural sciences
Autophagy-Related Protein 5
chemistry.chemical_compound
Nitrate
CHLOROPLASTS
Biomass
[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences
harvest index (HI)
Cellular Senescence
2. Zero hunger
0303 health sciences
biology
15N
food and beverages
nitrogen remobilization
Plants, Genetically Modified
Chloroplast
Phenotype
Seeds
RNA Interference
RIBULOSE-1
autophagy
Nitrogen
PROTEINS
Ribulose-Bisphosphate Carboxylase
03 medical and health sciences
Botany
LEAVES
LEAF SENESCENCE
Nitrogen cycle
030304 developmental biology
Nitrates
Nitrogen Isotopes
THALIANA
Arabidopsis Proteins
5-BISPHOSPHATE CARBOXYLASE/OXYGENASE
Autophagy
RuBisCO
DEGRADATION
yield
biology.organism_classification
RUBISCO
Carbon
Phosphoric Monoester Hydrolases
Plant Leaves
chemistry
Mutation
Vacuoles
biology.protein
nitrogen use efficiency (NUE)
STARVATION
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14698137 and 0028646X
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- New Phytologist
- Accession number :
- edsair.doi.dedup.....16e0d13c76f3b210a658986c99632de7