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Molecular typing and phenotype characterization of methicillin-resistant Staphylococcus aureus isolates from blood in Taiwan
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 1, p e30394 (2012)
- Publication Year :
- 2011
-
Abstract
- Background Staphylococcus aureus causes a variety of severe infections such as bacteremia and sepsis. At present, 60–80% of S. aureus isolates from Taiwan are methicillin resistant (MRSA). It has been shown that certain MRSA clones circulate worldwide. The goals of this study were to identify MRSA clones in Taiwan and to correlate the molecular types of isolates with their phenotypes. Methods A total of 157 MRSA isolates from bacteremic patients were collected from nine medical centers. They were typed based on polymorphisms in agr, SCCmec, MLST, spa, and dru. Phenotypes characterized included Panton-Valentine leucocidin (pvl), inducible macrolide-lincosamide-streptogramin B resistance (MLSBi), vancomycin (VA) and daptomycin (DAP) minimal inhibitory concentrations (MIC), and superantigenic toxin gene profiles. Difference between two consecutive samples was determined by Mann-Whitney-U test, and difference between two categorical variables was determined by Fisher's exact test. Results Four major MRSA clone complexes CC1, CC5, CC8, and CC59 were found, including 4 CC1, 9 CC5, 111 CC8, and 28 CC59 isolates. These clones had the following molecular types: CC1: SCCmecIV and ST573; CC5: SCCmecII and ST5; CC8: SCCmecIII, ST239, and ST241, and CC59: SCCmecIV, SCCmecVT, ST59, and ST338. The toxin gene profiles of these clones were CC1: sec-seg-(sei)-sell-selm-(seln)-selo; CC5: sec-seg-sei-sell-selm-(seln)-selp-tst1; CC8: sea-selk-selq, and CC59: seb-selk-selq. Most isolates with SCCmecVT, ST59, spat437, and dru11 types were pvl+ (13 isolates), while multidrug resistance (≥4 antimicrobials) were associated with SCCmecIII, ST239, spa t037, agrI, and dru14 (119 isolates) (p
- Subjects :
- DNA, Bacterial
Methicillin-Resistant Staphylococcus aureus
Bacterial Diseases
Heredity
Virulence Factors
DNA Mutational Analysis
Taiwan
lcsh:Medicine
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
Antibiotic resistance
medicine
Genetics
Humans
lcsh:Science
Antigens, Bacterial
Multidisciplinary
Polymorphism, Genetic
SCCmec
lcsh:R
biochemical phenomena, metabolism, and nutrition
Staphylococcal Infections
bacterial infections and mycoses
Virology
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Bacterial Pathogens
Multiple drug resistance
Molecular Typing
Phenotype
Infectious Diseases
Staphylococcus aureus
Vancomycin
Multilocus sequence typing
Medicine
lcsh:Q
Daptomycin
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....16f7bcf0213023cf3456402665004fb9