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Long noncoding RNA SNHG1 protects brain microvascular endothelial cells against oxygen–glucose deprivation/reoxygenation-induced injury by sponging miR-298 and upregulating SIK1 expression
- Source :
- Biotechnology Letters. 43:1163-1174
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Growing evidence shows that long non-coding RNAs (lncRNAs) are widely involved in the progression of multiple diseases, including ischemic stroke. The aim of this study was to explore the function and underlying mechanism of lncRNAs small nucleolar RNA host gene 1 (SNHG1) in ischemic stroke. SNHG1 and salt-induced kinase 1 (SIK1) were upregulated in oxygen–glucose deprivation/reperfusion (OGD/R)-induced bEnd3 cells. SNHG1 downregulation promoted OGD/R-induced injury through decreasing cell proliferation and increasing apoptosis, which was reversed by upregulating SIK1 or downregulating miR-298. Moreover, SIK1 interference had similar functions with SNHG1 knockdown in OGD/R-treated bEnd3 cells. In addition, miR-298 was a direct target of SNHG1 and could specifically bind to SIK1. Furthermore, SNHG1 functioned as a molecular sponge of miR-298 to regulate SIK1 expression. SNHG1 knockdown enhanced OGD/R-induced injury in bEnd3 cells by regulating miR-298/SIK1 axis, which might provide promising therapeutic target for treatment of ischemic stroke.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cell Survival
Bioengineering
Protein Serine-Threonine Kinases
Models, Biological
01 natural sciences
Applied Microbiology and Biotechnology
Cell Line
Mice
03 medical and health sciences
Downregulation and upregulation
010608 biotechnology
Animals
Small nucleolar RNA
Cell Proliferation
Ischemic Stroke
Gene knockdown
Cell growth
Chemistry
Kinase
Brain
Endothelial Cells
General Medicine
Long non-coding RNA
Up-Regulation
Cell biology
MicroRNAs
030104 developmental biology
Gene Expression Regulation
Apoptosis
Gene Knockdown Techniques
RNA, Long Noncoding
Function (biology)
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....171540b5d147c10cc51797bfa932f5dc