Additional file 1 of Discrepancy in p16 expression in patients with HPV-associated head and neck squamous cell carcinoma in Thailand: clinical characteristics and survival outcomes

Additional file 1: Supplement 1. Real-time PCR typing of HPV with AmoyDx High-risk Human Papillomavirus (HPV) Detection Kit. One reaction with three fluorescents (A:FAM-other 17 HPVs high risk, B: Cy5-HPV 16/18 and C: HEX-Internal control) were mixed in each reaction tube for identification of the 19 possible HPV high risk types in a real-time PCR setting, as described in the Material and Methods section (1: positive control, 2: no template control, 3: positive other 17 HPVs high risk sample, 4: positive HPV type 16 or 18 sample and 5: internal control of sample). Supplement 2. Baseline characteristics of non-oropharyngeal HNSCC and p16 status.