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Effects of Astragalus polysaccharide on immune responses of porcine PBMC stimulated with PRRSV or CSFV
- Source :
- PLoS ONE, Vol 7, Iss 1, p e29320 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Background Astragalus polysaccharide (APS) has been used as an immunomodulator that can enhance immune responses, whereas the immunomodulatory effects of APS on porcine peripheral blood mononuclear cells (PBMCs) exposed to porcine reproductive and respiratory syndrome virus (PRRSV) and classical swine fever virus (CSFV) have not been investigated. Methodology/Principal Findings Porcine PBMCs were cultured in complete RPMI media in the presence of the R98-strain of PRRSV (5×104 TCID50/ml) or C-strain of CSFV (103 TCID50/ml) with or without APS. The expression of mRNA for CD28, cytotoxic T-lymphocyte antigen 4 (CTLA-4), transforming growth factor-β (TGF-β), interleukin 2 (IL-2) and IL-10 was assayed by TaqMan real-time RT-PCR. The expression of mRNA for CD28 and CTLA-4 increased at 24 h after stimulation of PBMCs with CSFV and the increased production of CTLA-4 was confirmed by western blot analysis, whereas the increases were inhibited by the addition of APS. In addition, APS alone upregulated IL-2 and TGF-β mRNA expression in PBMCs and the addition of APS had the capacity to prevent a further increase in IL-2 mRNA expression in PBMCs during CSFV or PRRSV infection, but had no effect on TGF-β mRNA expression. The production of tumor necrosis factor-alpha (TNF-α) increased at 12 h after stimulation with PRRSV or CSFV, but not with PRRSV plus APS or CSFV plus APS, whereas the addition of APS to PBMCs infected with PRRSV or CSFV promoted IL-10 mRNA expression. Conclusions We suggested that APS had immunomodulatory effects on cells exposed to PRRSV or CSFV. It might be that APS via different mechanisms affects the activities of immune cells during either PRRSV or CSFV infection. This possibility warrants further studies to evaluate whether APS would be an effective adjuvant in vaccines against PRRSV or CSFV.
- Subjects :
- Proteomics
Veterinary Medicine
Interleukin 2
animal diseases
Sus scrofa
Immunology
lcsh:Medicine
Enzyme-Linked Immunosorbent Assay
Biology
Lymphocyte Activation
Peripheral blood mononuclear cell
Immune system
CD28 Antigens
Complementary and Alternative Medicine
Antigen
Polysaccharides
medicine
Animals
Cytotoxic T cell
CTLA-4 Antigen
Porcine respiratory and reproductive syndrome virus
RNA, Messenger
lcsh:Science
Cell Proliferation
Multidisciplinary
lcsh:R
Immunity
Computational Biology
virus diseases
CD28
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
Virology
Gene Expression Regulation
Classical Swine Fever Virus
Immune System
Leukocytes, Mononuclear
Immunologic Techniques
Cytokines
Medicine
Clinical Immunology
Veterinary Science
Tumor necrosis factor alpha
lcsh:Q
Subcellular Fractions
Research Article
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1736b53789b704cffe7a6fab13ad9307