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Improved sensitivity of lateral flow assay using paper-based sample concentration technique
- Source :
- Talanta. 152
- Publication Year :
- 2015
-
Abstract
- Lateral flow assays (LFAs) hold great promise for point-of-care testing, especially in resource-poor settings. However, the poor sensitivity of LFAs limits their widespread applications. To address this, we developed a novel device by integrating dialysis-based concentration method into LFAs. The device successfully achieved 10-fold signal enhancement in Human Immunodeficiency Virus (HIV) nucleic acid detection with a detection limit of 0.1 nM and 4-fold signal enhancement in myoglobin (MYO) detection with a detection limit of 1.56 ng/mL in less than 25 min. This simple, low-cost and portable integrated device holds great potential for highly sensitive detection of various target analytes for medical diagnostics, food safety analysis and environmental monitoring.
- Subjects :
- Analyte
Medical diagnostic
Point-of-Care Systems
Human immunodeficiency virus (HIV)
Metal Nanoparticles
02 engineering and technology
medicine.disease_cause
01 natural sciences
Analytical Chemistry
Limit of Detection
medicine
Humans
Detection limit
Chemistry
Myoglobin
010401 analytical chemistry
HIV
Paper based
021001 nanoscience & nanotechnology
0104 chemical sciences
Highly sensitive
RNA, Viral
Biological Assay
Gold
0210 nano-technology
Sensitivity (electronics)
Dialysis
Nucleic acid detection
Biomedical engineering
Subjects
Details
- ISSN :
- 18733573
- Volume :
- 152
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....173df53532f4d5b156a3e53c44a08723