Supplementary Figure 1 from T-cell Expression of IL10 Is Essential for Tumor Immune Surveillance in the Small Intestine

Representative analysis of bone marrow reconstitution efficiency. (A) FACS of total live MNC derived from the intestine of donor CD45.2+ mouse. (B) Frequencies of CD45.1+ and CD45.2+ cells in the intestine of a representative chimeric mouse. CD45.1 donor bone marrow cells were depleted of Lin+ cells and injected into retro-orbital sinus of lethally irradiated 2 month old CD45.2 APCdelta468 mice. To establish the level of bone marrow reconstitution, chimeric APCdelta468 mice were aged to 4 months of age and intestinal MNC were isolated and analyzed by flow cytometry for expression of CD45.2 and CD45.1.