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Piperacillin/tazobactum and cefotaxime decrease the effect of beta lactamase production in multi-drug resistant K. pneumoniae
- Source :
- Journal of Infection and Public Health, Vol 14, Iss 12, Pp 1777-1782 (2021)
- Publication Year :
- 2021
-
Abstract
- Background Worldwide, multi-drug resistant Klebsiella pneumoniae (K. pneumonia) and their virulence’s were contributed more in the multi-drug resistant effect. According to the World Health organization report, it is an emerging thread in developing countries and also comes under first ever critical list. In this context, the current study was concentrated on detection of extended spectrum beta lactamase (ESBL) producing strain and their antimicrobial susceptibility study of K. pneumoniae. Materials and methods Firstly, the multi-drug resistant effect of the K. pneumoniae was identified from specific CLSI guidelines recommended antibiotics by disc diffusion method. Consecutively, the primary ESBL identification test was performed using ceftazidime and cefotaxime, followed by double disc combination and phenotypic confirmation tests using ceftazidime/clavulanic acid and cefotaxime/clavulanic acid. Finally, the minimum inhibition concentration of some important sensitive antibiotics were performed against selected K. pneumoniae was confirmed by micro broth dilution method. Results and conclusions The current result was most favorable to selected K. pneumoniae with more multi drug resistant characteristic nature. All the performed antibiotics were almost more sensitive to selected K. pneumoniae. The effective antibiotics of piperacillin was also exhibited more resistant effect against tested bacteria and it cleaved the bacterial enzyme clearly. The present result of primary ESBL identification test result was exhibited with ≤22 mm and ≤27 mm against ceftazidime and cefotaxime were observed respectively. Followed result of double disc combination and phenotypic confirmation experiments results were clearly stated that the selected K. pneumoniae was ESBL producer. The ceftazidime, cefotaxime and ceftazidime/clavulanic acid and cefotaxime/clavulanic acid were exhibited with merged zones and ≥5 mm zones around the combination disc when compared with disc alone were observed. All the ESBL detection test results were clearly indicated that the selected K. pneumoniae strain was ESBL producer.
- Subjects :
- Cefotaxime
Antibiotic resistance
Klebsiella pneumoniae
medicine.drug_class
medicine.medical_treatment
Antibiotics
Ceftazidime
Context (language use)
Infectious and parasitic diseases
RC109-216
Microbial Sensitivity Tests
Biology
Phenotypic assay
beta-Lactamases
Microbiology
CLSI guidelines
Clavulanic acid
medicine
Humans
Piperacillin
Urinary tract infection
Public Health, Environmental and Occupational Health
General Medicine
biology.organism_classification
Anti-Bacterial Agents
Infectious Diseases
Gene identification
Pharmaceutical Preparations
Beta-lactamase
Public aspects of medicine
RA1-1270
Gram negative bacteria
medicine.drug
Subjects
Details
- ISSN :
- 1876035X
- Volume :
- 14
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of infection and public health
- Accession number :
- edsair.doi.dedup.....17759a91a7c8293d700dce460cd2cb12