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Therapeutic Effects of Insulin-Producing Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Type 1 Diabetes Mouse Model
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 13; Pages: 6877
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- In patients with type 1 diabetes (T1D), compromised pancreatic β-cell functions are compensated through daily insulin injections or the transplantation of pancreatic tissue or islet cells. However, both approaches are associated with specific challenges. The transplantation of mesenchymal stem cells (MSCs) represents a potential alternative, as MSCs have tissue-forming capacity and can be isolated from various tissues. The human umbilical cord (hUC) is a good source of freely available MSCs, which can be collected through pain-free, non-invasive methods subject to minimal ethical concerns. We sought to develop a method for the in vitro generation of insulin-producing cells (IPCs) using MSCs. We examined the potential therapeutic uses and efficacy of IPCs generated from hUC-derived MSCs (hUC-IPCs) and human adipose tissue (hAD)-derived MSCs (hAD-IPCs) through in vitro experiments and streptozotocin (STZ)-induced C57BL/6 T1D mouse models. We discovered that compared to hAD-IPCs, hUC-IPCs exhibited a superior insulin secretion capacity. Therefore, hUC-IPCs were selected as candidates for T1D cell therapy in mice. Fasting glucose and intraperitoneal glucose tolerance test levels were lower in hUC-IPC-transplanted mice than in T1D control mice and hAD-IPC-transplanted mice. Our findings support the potential use of MSCs for the treatment of T1D.
- Subjects :
- endocrine system diseases
Organic Chemistry
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
Mesenchymal Stem Cell Transplantation
Catalysis
Umbilical Cord
Computer Science Applications
Mice, Inbred C57BL
type 1 diabetes
mesenchymal stem cells
insulin-producing cells
transplantation
direct-trans differentiation
Inorganic Chemistry
Mice
Diabetes Mellitus, Type 1
parasitic diseases
Animals
Humans
Insulin
cardiovascular diseases
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....17905886f53992c076bf56d3c60a6012