Back to Search Start Over

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

Authors :
Jung Seung Lee
Yun-Gon Kim
Hoon Chul Kang
Ji Hun Kim
Ann Na Cho
Dong-Jun Koo
Jin Kim
Yi Sun Choi
Ju-Young Kim
Yoonhee Jin
Weonjin Yu
Sung-Hyun Jo
Gyeong-Eon Chang
Nakwon Choi
Won-Young Choi
Sung-Yon Kim
Seung Woo Cho
Dongyoon Kim
Eunji Cheong
Jung-Hoon Kim
Yeonjoo An
Hyunsoo Shawn Je
Young Joon Kim
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-23 (2021), Nature Communications
Publication Year :
2021
Publisher :
Springer Science and Business Media LLC, 2021.

Abstract

Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.<br />Brain organoids derived from human pluripotent stem cells can model human brain development and disease, though current culture systems fail to ensure reliable production of high-quality organoids. Here the authors combine human brain extracellular matrix and culture in a microfluidic device to promote structural and functional maturation of human brain organoids.

Details

ISSN :
20411723
Volume :
12
Database :
OpenAIRE
Journal :
Nature Communications
Accession number :
edsair.doi.dedup.....17d17756bbb501d0fa9774153109b50c
Full Text :
https://doi.org/10.1038/s41467-021-24775-5