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Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
- Source :
- Nature Communications, Vol 12, Iss 1, Pp 1-23 (2021), Nature Communications
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.<br />Brain organoids derived from human pluripotent stem cells can model human brain development and disease, though current culture systems fail to ensure reliable production of high-quality organoids. Here the authors combine human brain extracellular matrix and culture in a microfluidic device to promote structural and functional maturation of human brain organoids.
- Subjects :
- Models, Anatomic
Swine
Neurogenesis
Science
Microfluidics
Induced Pluripotent Stem Cells
Models, Neurological
General Physics and Astronomy
Biology
Article
Stem-cell biotechnology
General Biochemistry, Genetics and Molecular Biology
Biomaterials
Extracellular matrix
Organ Culture Techniques
Lab-On-A-Chip Devices
Organoid
medicine
Animals
Humans
Tissue engineering
Induced pluripotent stem cell
Multidisciplinary
Gene Expression Profiling
Brain
Hydrogels
General Chemistry
Human brain
Periodic flow
Culture Media
Electrophysiological Phenomena
Extracellular Matrix
Cell biology
Organoids
medicine.anatomical_structure
Microfluidic chamber
Feasibility Studies
Neuroglia
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....17d17756bbb501d0fa9774153109b50c
- Full Text :
- https://doi.org/10.1038/s41467-021-24775-5