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A High-Fat Diet Decreases Bone Mass in Growing Mice with Systemic Chronic Inflammation Induced by Low-Dose, Slow-Release Lipopolysaccharide Pellets
- Source :
- The Journal of nutrition. 147(10)
- Publication Year :
- 2017
-
Abstract
- Background: Chronic inflammation is associated with increased bone resorption and is linked to osteopenia, or low bone mass. Obesity is also associated with low-grade chronic upregulation of inflammatory cytokines.Objective: This study investigated the effect of high-fat (HF) diet-induced obesity on bone structure changes in growing mice with existing systemic chronic inflammation induced by low-dose, slow-release lipopolysaccharide (LPS).Methods: Forty-eight 6-wk-old female C57BL/6 mice were randomly assigned to 4 treatment groups (n = 12/group) in a 2 × 2 factorial design-control (placebo) or LPS treatment (1.5 μg/d)-and consumed either a normal-fat (NF, 10% of energy as fat) or an HF (45% of energy as fat) diet ad libitum for 13 wk. Bone structure, serum biomarkers of bone turnover, and osteoclast differentiation were measured.Results: No alterations were observed in final body weights, fat mass, or lean mass in response to LPS treatment. LPS treatment increased serum concentration of tartrate-resistant acid phosphatase (TRAP, a bone resorption marker) and bone marrow osteoclast differentiation and decreased femoral and lumbar vertebral bone volume (BV):total volume (TV) by 25% and 24%, respectively, compared with the placebo. Mice fed the HF diet had greater body weight at the end of the study (P < 0.01) due to increased fat mass (P < 0.01) than did mice fed the NF diet. The HF diet increased serum TRAP concentration, bone marrow osteoclast differentiation, and expression of tumor necrosis factor α, interleukin 1β and interleukin 6 in adipose tissue. Compared with the NF diet, the HF diet decreased BV:TV by 10% and 8% at femur and lumbar vertebrae, respectively, and the HF diet was detrimental to femoral and lumbar vertebral bone structure with decreased trabecular number and increased trabecular separation and structure model index.Conclusion: Results suggest that HF diets and systemic chronic inflammation have independent negative effects on bone structure in mice.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
medicine.medical_specialty
Interleukin-1beta
Medicine (miscellaneous)
Adipose tissue
Osteoclasts
Inflammation
Diet, High-Fat
Bone resorption
Bone and Bones
Bone remodeling
03 medical and health sciences
Mice
Random Allocation
Osteoclast
Bone Density
Internal medicine
medicine
Animals
Femur
Obesity
Nutrition and Dietetics
Lumbar Vertebrae
business.industry
Interleukin-6
Tartrate-Resistant Acid Phosphatase
Tumor Necrosis Factor-alpha
Cell Differentiation
medicine.disease
Dietary Fats
Osteopenia
Mice, Inbred C57BL
Bone Diseases, Metabolic
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Animals, Newborn
Lean body mass
Female
Bone marrow
Bone Remodeling
medicine.symptom
business
Biomarkers
Subjects
Details
- ISSN :
- 15416100
- Volume :
- 147
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The Journal of nutrition
- Accession number :
- edsair.doi.dedup.....17d6e3f7ed5de94c5801d1f43b79e88e