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Transcriptomic analysis of equine placenta reveals key regulators and pathways involved in ascending placentitisâ€
- Source :
- Biology of Reproduction. 104:638-656
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Improved understanding of the molecular mechanisms underlying ascending equine placentitis holds the potential for the development of new diagnostic tools and therapies to forestall placentitis-induced preterm labor. The current study characterized the equine placental transcriptome (chorioallantois [CA] and endometrium [EN]) during placentitis (placentitis group, n = 6) in comparison to gestationally-matched controls (control group, n = 6). Transcriptome analysis identified 2953 and 805 differentially expressed genes in CA and EN during placentitis, respectively. Upstream regulator analysis revealed the central role of toll-like receptors (TLRs) in triggering the inflammatory signaling, and consequent immune-cell chemotaxis. Placentitis was associated with the upregulation of matrix metalloproteinase (MMP1, MMP2, and MMP9) and apoptosis-related genes such as caspases (CASP3, CASP4, and CASP7) in CA. Also, placentitis was associated with downregulation of transcripts coding for proteins essential for placental steroidogenesis (SRD5A1 and AKR1C1), progestin signaling (PGRMC1 and PXR) angiogenesis (VEGFA, VEGFR2, and VEGFR3), and nutrient transport (GLUT12 and SLC1A4), as well as upregulation of hypoxia-related genes (HIF1A and EGLN3), which could explain placental insufficiency during placentitis. Placentitis was also associated with aberrant expression of several placenta-regulatory genes, such as PLAC8, PAPPA, LGALS1, ABCG2, GCM1, and TEPP, which could negatively affect placental functions. In conclusion, our findings revealed for the first time the key regulators and mechanisms underlying placental inflammation, separation, and insufficiency during equine placentitis, which might lead to the development of efficacious therapies or diagnostic aids by targeting the key molecular pathways.Summary sentenceThis is the first study to characterize the equine placenta transcriptome to identify the key regulators and pathways underlying placental inflammation, separation, and insufficiency during the equine ascending placentitis.Graphical Abstract
- Subjects :
- 0301 basic medicine
Placenta Diseases
MMP1
Angiogenesis
Placenta
Down-Regulation
Placental insufficiency
Biology
MMP9
Transcriptome
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Pregnancy
Streptococcal Infections
medicine
Animals
Streptococcus equi
Horses
030219 obstetrics & reproductive medicine
Cell Biology
General Medicine
medicine.disease
Immunohistochemistry
Up-Regulation
Cell biology
030104 developmental biology
HIF1A
medicine.anatomical_structure
Gene Expression Regulation
Reproductive Medicine
Female
Horse Diseases
Subjects
Details
- ISSN :
- 15297268 and 00063363
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Biology of Reproduction
- Accession number :
- edsair.doi.dedup.....17e187f1c687fefff3b44bbf2417f032