Additional file 1 of All-in-one theranostic nano-platform based on polymer nanoparticles for BRET/FRET-initiated bioluminescence imaging and synergistically anti-inflammatory therapy for ulcerative colitis

Additional file 1: Fig. S1. P-selectin expressions in clinical samples from chronic colitis and adenocarcinoma patients. A human P-selectin antibody was used to observe the expression level of P-selectin in inflammatory site of colon and cancer tissues via a human tissue microarray counterstained with hematoxylin. Scale bar: 20 ��m. Fig. S2. Cellular uptake of BA/Res@NP and BA/Res@NP-PBP by colon-26 cells (A) and Raw 264.7 cells (B). The cell nucleus was stained by DAPI (Blue), cytoskeleton was stained by FITC-phalloidin (Green), and NPs were labeled by lipophilic carbocyanine dye, DiL (Red), scale bar: 20 ��m. Fig. S3. In vitro biocompatibility of Blank@NPs. Cell vitablity of Colon-26 cells after being incubated with Blank@NPs compared with Control at varied concentrations (0, 10, 20, 50, 100, 200, and 400 ��g/ mL) for 24 h (A) and 48 h (B). Fig. S4. Changes of body weight over time in Control group and Blank@NP-treated group. Fig. S5. In vivo toxicity was evaluated by H&E staining of vital organ tissues. The organs were harvested, fixed in 10% formalin, embedded in paraffin, sectioned, subjected to H&E staining, and examined for histological assessment. Representative images are shown (n=3). Scale bar: 50 ��m. Fig. S6. Partial blood test (A) and biochemical parameters (B) of mice in the Control group and Blank@NP-treated group. Each point represents the mean �� SEM (n=3). Fig. S7. Colon length in different groups of mice. Fig. S8. Histopathologic score in different groups of mice. Table S1. Primers used for Real-time PCR. Table S2. The entrapment efficiency and loading capacity of NPs doped with different BA/Res ratios. Experiment methods: Staining of colon microarrays; Cell apoptosis study; In vivo monitoring of inflammation during acute UC; Histological analyses of tissue sections by hematoxylin and eosin staining; Impact of BA/Res@NP on intestinal microbiota.