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Beta adrenergic receptors in human cavernous tissue
- Source :
- The Journal of urology. 133(4)
- Publication Year :
- 1985
-
Abstract
- Beta adrenergic receptor binding was performed with 125I iodocyanopindolol on human cavernous tissue membrane fractions from normal tissue and transsexual procedures obtained postoperatively, as well as from postmortem sources. Isotherm binding studies on normal fresh tissues indicated that the receptor density was 9.1 fmoles/mg. with a KD of 23 pM. Tissue stored at room temperature for 4 to 6 hours, then at 4C in saline solution for 19 to 20 hours before freezing showed no significant changes in receptor density or affinity, and provided evidence for the stability of postmortem tissue obtained within the same time period. Beta receptor density of 2 cavernous preparations from transsexual procedures was not significantly different from normal control tissues, and showed that high concentrations of estrogen received by these patients had no effect on beta adrenergic receptor density. Displacement of 125iodocyanopindolol by 5 beta adrenergic agents demonstrated that 1-propranolol had the greatest affinity followed by ICI 118,551, zinterol, metoprolol and practolol. When the results of these displacement studies were subjected to Scatfit, non-linear regression line analysis, a single binding site was described. Based on the relative potency of the selective beta adrenergic agents it appears that these receptors were of the beta 2 subtype.
- Subjects :
- Male
medicine.medical_specialty
Adrenergic receptor
Urology
Adrenergic beta-Antagonists
Iodocyanopindolol
Iodine Radioisotopes
Propanolamines
chemistry.chemical_compound
Internal medicine
Receptors, Adrenergic, beta
Medicine
Humans
Sympatholytics
Zinterol
Receptor
Beta (finance)
Practolol
Binding Sites
business.industry
Adrenergic beta-Agonists
Propranolol
medicine.anatomical_structure
Endocrinology
chemistry
Cavernous tissue
Ethanolamines
Pindolol
business
medicine.drug
Metoprolol
Penis
Subjects
Details
- ISSN :
- 00225347
- Volume :
- 133
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Journal of urology
- Accession number :
- edsair.doi.dedup.....17fd4e03cd0223539e6ed2e9b48bd610