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Sodium thiosulfate enhances production of polysaccharides and anticancer activities of sulfated polysaccharides in Antrodia cinnamomea
- Source :
- Carbohydrate Polymers. 216:204-212
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Sulfated polysaccharides (SPSs) are polysaccharides (PSs) with high sulfate functionalization and possess bioactivities. This study aimed to increase the sulfate content of SPSs in Antrodia cinnamomea through sulfate feeding. Feeding A. cinnamomea with sodium thiosulfate was found to increase yields of PSs and SPSs in A. cinnamomea. The SPSs thus obtained (ST-SPS) were further isolated, showing enhanced sulfate content of 2.5 mmol/g. Sodium thiosulfate induced changes in molecular weight from 320 kDa to 1342 kDa, and area percentage of low-molecular-weight ST-SPS (20 kDa) was decreased. Functional studies revealed that sodium thiosulfate increased the ST-SPS anticancer efficacy in cancer cells via inhibition of EGFR/AKT signaling. Moreover, the ST-SPS enhanced synergistically cisplatin-, gefitinib- and 5 FU-induced cytotoxic effects in lung cancer H1975 cells and colon cancer CT26 cells. This study is the first to demonstrate that sodium thiosulfate induced changes in properties of A. cinnamomea with the anticancer mechanisms of ST-SPS.
- Subjects :
- Polymers and Plastics
education
Thiosulfates
Antineoplastic Agents
Apoptosis
02 engineering and technology
Sulfuric Acid Esters
Sodium thiosulfate
010402 general chemistry
Polysaccharide
01 natural sciences
Inhibitory Concentration 50
Mice
chemistry.chemical_compound
Polysaccharides
Cell Line, Tumor
Materials Chemistry
Animals
Humans
Food science
Phosphorylation
Sulfate
chemistry.chemical_classification
Chemistry
Organic Chemistry
Drug Synergism
Gefitinib
021001 nanoscience & nanotechnology
0104 chemical sciences
ErbB Receptors
Molecular Weight
Antrodia
Sulfated polysaccharides
Fluorouracil
Cisplatin
0210 nano-technology
Antrodia cinnamomea
Signal Transduction
Subjects
Details
- ISSN :
- 01448617
- Volume :
- 216
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Polymers
- Accession number :
- edsair.doi.dedup.....18083678edca23fde288daa5b2291021