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Endurance training limits the functional alterations of heart rat mitochondria submitted to in vitro anoxia-reoxygenation
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2006
-
Abstract
- Background Studies analysing the effect of endurance training on heart mitochondrial function submitted to in vitro anoxia-reoxygenation (A-R) are missing. The present study aimed to investigate the effect of moderate endurance treadmill training (14 weeks) against rat heart mitochondrial dysfunction induced by in vitro A-R. Methods Respiratory parameters (state 3, state 4, ADP/O and respiratory control ratio—RCR) and oxidative damage markers (carbonyl groups and malondialdehyde) were determined in isolated mitochondria before and after 1 min anoxia followed by 4 min reoxygenation. Levels of heat shock protein 60 kDa (HSP60) and 70 kDa (HSP70) were measured before A-R in mitochondria and whole muscle homogenate, respectively. Results A-R significantly impaired the rate of state 3 and state 4 respiration, as well as the RCR and ADP/O in the sedentary group. However, mitochondrial state 3 respiration was significantly higher in trained than in the sedentary group both before and after A-R. The impairments in RCR, ADP/O ratio and state 4 induced by A-R in sedentary group were significantly attenuated in endurance-trained group. The inhibition of state 4 induced by GDP was significantly higher in trained than in sedentary group. Oxidative modifications of mitochondrial proteins and phospholipids were found in sedentary group after A-R, although limited in trained group. Increased levels of mitochondrial HSP60 and tissue HSP70 accompanied the lower decrease in the respiratory function after A-R observed in trained group. Conclusion We therefore concluded that endurance training limited the impairments on rat heart mitochondria caused by the oxidant insult inflicted by in vitro A-R.
- Subjects :
- Male
medicine.medical_specialty
Cellular respiration
Cell Respiration
Outras ciências médicas
Citrate (si)-Synthase
Oxidative phosphorylation
Mitochondrion
Mitochondria, Heart
Oxidative Phosphorylation
Protein Carbonylation
chemistry.chemical_compound
Oxygen Consumption
Other medical sciences
Endurance training
Physical Conditioning, Animal
Internal medicine
Respiration
Oxidative damage
medicine
Animals
HSP70 Heat-Shock Proteins
Respiratory function
Rats, Wistar
Hypoxia
Exercise
Heart metabolism
Heat shock proteins
business.industry
Chaperonin 60
Other medical sciences [Medical and Health sciences]
Malondialdehyde
Rats
Surgery
Disease Models, Animal
Endocrinology
chemistry
Cardiac mitochondria
Exercise Test
Physical Endurance
Lipid Peroxidation
Cardiology and Cardiovascular Medicine
business
Outras ciências médicas [Ciências médicas e da saúde]
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....1819096981a36544adae897eaf7a8a84