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PD-1 Expression and Cytokine Secretion Profiles of Mycobacterium tuberculosis-Specific CD4+ T-Cell Subsets; Potential Correlates of Containment in HIV-TB Co-Infection
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 1, p e0146905 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- HIV co-infection is an important risk factor for tuberculosis (TB) providing a powerful model in which to dissect out defective, protective and dysfunctional Mycobacterium tuberculosis (MTB)-specific immune responses. To identify the changes induced by HIV co-infection we compared MTB-specific CD4+ responses in subjects with active TB and latent TB infection (LTBI), with and without HIV co-infection. CD4+ T-cell subsets producing interferon-gamma (IFN-γ), interleukin-2 (IL-2) and tumour necrosis factor-alpha (TNF-α) and expressing CD279 (PD-1) were measured using polychromatic flow-cytometry. HIV-TB co-infection was consistently and independently associated with a reduced frequency of CD4+ IFN-γ and IL-2-dual secreting T-cells and the proportion correlated inversely with HIV viral load (VL). The impact of HIV co-infection on this key MTB-specific T-cell subset identifies them as a potential correlate of mycobacterial immune containment. The percentage of MTB-specific IFN-γ-secreting T-cell subsets that expressed PD-1 was increased in active TB with HIV co-infection and correlated with VL. This identifies a novel correlate of dysregulated immunity to MTB, which may in part explain the paucity of inflammatory response in the face of mycobacterial dissemination that characterizes active TB with HIV co-infection.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes
Male
0301 basic medicine
Interleukin 2
Tuberculosis
General Science & Technology
Programmed Cell Death 1 Receptor
lcsh:Medicine
HIV Infections
Biology
Immunophenotyping
Mycobacterium tuberculosis
Interferon-gamma
Young Adult
03 medical and health sciences
Immune system
Latent Tuberculosis
MD Multidisciplinary
medicine
Humans
lcsh:Science
Antigens, Bacterial
Multidisciplinary
Latent tuberculosis
Coinfection
Tumor Necrosis Factor-alpha
lcsh:R
Middle Aged
medicine.disease
biology.organism_classification
Virology
Lymphocyte Subsets
3. Good health
030104 developmental biology
Gene Expression Regulation
Immunology
Interleukin-2
lcsh:Q
Female
Cytokine secretion
Viral load
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....1889bdfd8efed0b45fa4f68385e87bb8