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Purification of Crude Fructo-Oligosaccharide Preparations Using Probiotic Bacteria for the Selective Fermentation of Monosaccharide Byproducts
- Source :
- Frontiers in Microbiology, Vol 11 (2021), Frontiers in Microbiology
- Publication Year :
- 2021
-
Abstract
- Probiotics are microbes that promote health when consumed in sufficient amounts. They are present in many fermented foods or can be provided directly as supplements. Probiotics utilize non-digestible prebiotic oligosaccharides for growth in the intestinal tract, contributing to a healthy microbiome. The oligosaccharides favored by probiotics are species-dependent, as shown by the selective utilization of substrates in mixed sugar solutions such as crude fructo-oligosaccharides (FOS). Enzymatically produced crude FOS preparations contain abundant monosaccharide byproducts, residual sucrose, and FOS varying in chain length. Here we investigated the metabolic profiles of four probiotic bacteria during the batch fermentation of crude FOS under controlled conditions. We found that Bacillus subtilis rapidly utilized most of the monosaccharides but little sucrose or FOS. We therefore tested the feasibility of a microbial fed-batch fermentation process for the purification of FOS from crude preparations, which increased the purity of FOS from 59.2 to 82.5% with a final concentration of 140 g·l−1. We also tested cell immobilization in alginate beads as a means to remove monosaccharides from crude FOS. This encapsulation concept establishes the basis for new synbiotic formulations that combine probiotic microbes and prebiotic oligosaccharides.
- Subjects :
- 0106 biological sciences
Microbiology (medical)
Sucrose
Synbiotics
medicine.medical_treatment
lcsh:QR1-502
fructo-oligosaccharides purification
01 natural sciences
Microbiology
lcsh:Microbiology
law.invention
Probiotic
chemistry.chemical_compound
0404 agricultural biotechnology
law
010608 biotechnology
sugar metabolism
medicine
Monosaccharide
Food science
synbiotics
Sugar
probiotic microorganism
Original Research
chemistry.chemical_classification
Prebiotic
04 agricultural and veterinary sciences
Oligosaccharide
040401 food science
chemistry
Fermentation
prebiotics
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology, Vol 11 (2021), Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....188c7d69b38abc00ff9caebc1ca691fe