Highly efficient enzymatic synthesis of 3'-deoxyapionucleic acid (apioNA) having the four natural nucleobases

The synthesis of the 3′-deoxyapionucleoside 3′′-triphosphates (apioNTPs) having the four natural nucleobases and their enzymatic incorporation into a DNA–DNA primer–template have been tried. Therminator DNA polymerase was shown to incorporate these apioNTPs effectively giving 43mer DNA–apioNA chimera.