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Ultrasensitive immunosensor for prostate specific antigen using biomimetic polydopamine nanospheres as an electrochemiluminescence superquencher and antibody carriers
- Source :
- Analytica Chimica Acta. 963:17-23
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- In this work, biomimetic polydopamine nanospheres (PDANSs) were easily prepared and were firstly exploited as an electrochemiluminescence (ECL) nanoquencher for the development of a sandwich type immunosensor. The PDANSs with abundant active functional groups can facilely label the detection antibody and show remarkable quenching effect towards the ECL of the tris-(2,2'-bipyridine)ruthenium (Ru(bpy)32+). The amino-modified multiwall carbon nanotubes/Nafion (MWCNTs-NH2@N) composite-film was adopted as a matrix to incorporate the luminophor Ru(bpy)32+ and immobilize the capture antibody. The prominent decrease of ECL signal intensity was obtained on account of the unique quenching ability of the labeled PDANSs. The quenching mechanism is believed that the excited states of Ru(bpy)32+ can be annihilated by quinone units in PDANSs via energy transfer. The ECL quenching efficiency was logarithmically related to the concentration of the prostate specific antigen (PSA) in the range from 0.1 pg mL-1 to 20 ng mL-1 with a detection limit of 35 fg mL-1. Furthermore, the proposed ECL immunosensor presented good stability, repeatability and selectivity.
- Subjects :
- Indoles
Polymers
chemistry.chemical_element
Nanotechnology
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Analytical Chemistry
Bipyridine
chemistry.chemical_compound
Biomimetic Materials
Limit of Detection
Nafion
Organometallic Compounds
Humans
Environmental Chemistry
Electrochemiluminescence
Spectroscopy
Immunoassay
Detection limit
Quenching (fluorescence)
Nanotubes, Carbon
Prostate-Specific Antigen
021001 nanoscience & nanotechnology
Combinatorial chemistry
0104 chemical sciences
Ruthenium
Quinone
Fluorocarbon Polymers
chemistry
Luminescent Measurements
0210 nano-technology
Selectivity
Antibodies, Immobilized
Nanospheres
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 963
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....18b199dd2b5f439cc4f42ff1556d7854