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Effects of phenylbutazone alone or in combination with a nutritional therapeutic on gastric ulcers, intestinal permeability, and fecal microbiota in horses
- Source :
- Journal of Veterinary Internal Medicine, Vol 35, Iss 2, Pp 1121-1130 (2021), Journal of Veterinary Internal Medicine
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background Gastrointestinal (GI) injury and dysbiosis are adverse events associated with nonsteroidal anti-inflammatory drug (NSAID) use in horses. Phenylbutazone has been shown to alter GI barrier function both in vitro and ex vivo, but its effects on barrier function have not been assessed in vivo. In addition, the ability of nutritional therapeutics to prevent these changes is not known. Objective Our objectives were to determine whether (a) phenylbutazone affected barrier function in vivo and (b) if phenylbutazone-induced GI injury could be ameliorated by the use of a nutritional therapeutic. Animals Thirty healthy horses were randomly assigned to 3 groups (n = 10 per group): control, phenylbutazone, or phenylbutazone plus nutritional therapeutic. Methods This study was conducted as a blinded, randomized block design. All horses were managed identically throughout the study period. Samples were collected throughout the study period to monitor fecal microbiota changes and gastric ulcers before and after treatment. Quantification of the bacterial 16S rRNA gene in blood was used as a marker of intestinal permeability. Results Phenylbutazone increased amounts of bacterial 16S rDNA in circulation 3.02-fold (95% confidence interval [CI], 0.1.89-4.17), increased gastric ulceration score by a mean of 1.1 grade (P = .02), and induced specific changes in the microbiota, including loss of Pseudobutyrivibrio of family Lachnospiraceae. These changes were attenuated by nutritional treatment. Conclusions and clinical importance Collectively, these findings suggest that phenylbutazone induces GI injury, including impaired barrier function, and that nutritional treatment could attenuate these changes.
- Subjects :
- medicine.medical_specialty
040301 veterinary sciences
NSAIDs
Inflammation
Standard Article
030204 cardiovascular system & hematology
Gastroenterology
Permeability
0403 veterinary science
03 medical and health sciences
0302 clinical medicine
In vivo
Internal medicine
RNA, Ribosomal, 16S
medicine
Phenylbutazone
Animals
gastric ulcers
Stomach Ulcer
Adverse effect
Barrier function
horses
Intestinal permeability
lcsh:Veterinary medicine
General Veterinary
business.industry
Microbiota
Anti-Inflammatory Agents, Non-Steroidal
04 agricultural and veterinary sciences
medicine.disease
Standard Articles
EQUINE
inflammation
lcsh:SF600-1100
Horse Diseases
medicine.symptom
business
barrier function
Dysbiosis
Ex vivo
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 08916640 and 19391676
- Volume :
- 35
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Internal Medicine
- Accession number :
- edsair.doi.dedup.....18e079fdd83f7100fa36a8308fac4325