Back to Search
Start Over
Effect of oxygen concentration and selected protocol factors on viability and gene expression of mouse liver slices
- Source :
- Toxicology in Vitro, 27(5), 1513-1524, Toxicology in Vitro 27 (2013) 5
- Publication Year :
- 2012
-
Abstract
- Precision cut liver slices (PCLSs) are widely used as a model to study hepatotoxicity. For culturing of PCLS diverse protocols are used which could affect slices viability and results. We aimed to identify the most optimal culture protocol for mouse PCLS. Slices were cultured for 24 h under different concentrations of serum, glucose, insulin, and oxygen. Thereafter, slices viability was assessed by biochemical methods. Transcriptome analysis was performed to identify changes introduced by culture at different oxygen concentrations (20%, 40%, 60%, and 80% of oxygen). Medium composition did not affect the slices viability. Although metabolic competence was unaffected by oxygen concentrations, culturing at 80% of oxygen yielded slices with the best biochemical characteristics. The comparison of uncultured vs. cultured slices revealed 2524 genes to be differentially expressed. Genes involved in drug metabolism, peroxisomal and mitochondrial functions were down-regulated while several adaptive/stress response processes were up-regulated. Moreover, 80% of oxygen was the most favorable condition with respect to maintenance of expression of genes involved in drug and energy metabolism. The outcome of this study indicates that mouse PCLS are a valuable tool in research on hepatic functions and toxicity, particularly if they are cultured under a controlled oxygen concentration of 80%.
- Subjects :
- Male
Novel Foods & Agrochains
BU Toxicologie
Cell Survival
chemistry.chemical_element
Biology
Novel Foods & Agroketens
Toxicology
Oxygen
Transcriptome
Bile Acids and Salts
Tissue Culture Techniques
Mice
Adenosine Triphosphate
Gene expression
Animals
Testosterone
BU Toxicology, Novel Foods & Agrochains
glucose
Toxicologie
Triglycerides
Oligonucleotide Array Sequence Analysis
precision-cut liver
L-Lactate Dehydrogenase
Gene Expression Profiling
BU Toxicology
toxicity
General Medicine
Metabolism
Peroxisome
tension
culture
Mice, Inbred C57BL
Biochemistry
chemistry
BU Toxicologie, Novel Foods & Agroketens
Liver
toxicogenomics
Toxicity
cells
systems
Limiting oxygen concentration
activation
Rikilt B&T Toxicologie en Effectanalyse
metabolism
Drug metabolism
Subjects
Details
- ISSN :
- 18793177 and 08872333
- Volume :
- 27
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Accession number :
- edsair.doi.dedup.....18f90df0746095dbd2d1fe2d50b06e2f