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IL-15 Improves the Cytotoxicity of Cytokine-induced Killer Cells Against Leukemia Cells by Upregulating CD3+CD56+ Cells and Downregulating Regulatory T Cells As Well As IL-35
- Source :
- Journal of Immunotherapy. 36:462-467
- Publication Year :
- 2013
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2013.
-
Abstract
- Cytokine-induced killer (CIK) cells are usually generated from peripheral blood mononuclear cells with the stimulation of IL-2 in vitro. Unlike the conventional IL-2-stimulated CIK cells (IL-2-CIK cells), we investigated the characteristics and potential mechanism of IL-15-stimulated CIK cells (IL-15-CIK cells) in this study. Compared with IL-2-CIK cells, the percentage of CD3CD56 cells was significantly increased in IL-15-CIK cells, but the expression of regulatory T (Treg) cells and IL-35 was significantly decreased in IL-15-CIK cells. Meanwhile, the in vitro cytotoxicity against human myeloid leukemia cells K562 of IL-15-CIK cells was significantly augmented compared with IL-2-CIK cells. These data suggest that IL-15 may improve the cytotoxicity of CIK cells against leukemia cells by upregulating CD3CD56 cells and downregulating Treg cells and IL-35.
- Subjects :
- Adult
Cytotoxicity, Immunologic
Cancer Research
CD3 Complex
Immunology
Down-Regulation
Gene Expression
T-Lymphocytes, Regulatory
Interleukin 21
Cytokine-Induced Killer Cells
Humans
Immunology and Allergy
IL-2 receptor
Antigen-presenting cell
Cells, Cultured
Interleukin 3
Interleukin-15
Pharmacology
Leukemia
CD40
biology
Cytokine-induced killer cell
Chemistry
Interleukins
Forkhead Transcription Factors
Natural killer T cell
CD56 Antigen
Up-Regulation
biology.protein
Cancer research
Interleukin 12
Interleukin-2
Natural Killer T-Cells
K562 Cells
Subjects
Details
- ISSN :
- 15249557
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Journal of Immunotherapy
- Accession number :
- edsair.doi.dedup.....1912d644a41b75b1e2c036f8356ef9d9