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Efficient monitoring of the blood-stage infection in a malaria rodent model by the rotating-crystal magneto-optical method
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Intense research efforts have been focused on the improvement of the efficiency and sensitivity of malaria diagnostics, especially in resource-limited settings for the detection of asymptomatic infections. Our recently developed magneto-optical (MO) method allows the accurate quantification of malaria pigment crystals (hemozoin) in blood by their magnetically induced rotation. First evaluations of the method using β-hematin crystals and in vitro P. falciparum cultures implied its potential for high-sensitivity malaria diagnosis. To further investigate this potential, here we study the performance of the method in monitoring the in vivo onset and progression of the blood-stage infection in a rodent malaria model. Our results show that the MO method can detect the first generation of intraerythrocytic P. berghei parasites 66–76 hours after sporozoite injection, demonstrating similar sensitivity to Giesma-stained light microscopy and exceeding that of flow cytometric techniques. Magneto-optical measurements performed during and after the treatment of P. berghei infections revealed that both the follow up under treatment and the detection of later reinfections are feasible with this new technique. The present study demonstrates that the MO method – besides being label and reagent-free, automated and rapid – has a high in vivo sensitivity and is ready for in-field evaluation.
- Subjects :
- 0301 basic medicine
Hemeproteins
Plasmodium berghei
030231 tropical medicine
Protozoan Proteins
Biology
Real-Time Polymerase Chain Reaction
Article
Magneto optical
03 medical and health sciences
Mice
0302 clinical medicine
In vivo
Microscopy
parasitic diseases
Anopheles
medicine
Animals
ddc:530
Life Cycle Stages
Mice, Inbred BALB C
Multidisciplinary
Hemozoin
Rodent model
DNA, Protozoan
medicine.disease
biology.organism_classification
Flow Cytometry
Malaria
Disease Models, Animal
030104 developmental biology
Immunology
Female
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....196c5ed212d7dbcbab9cd18ae68f8eb3