Back to Search
Start Over
Selective control of Cu(II) complex stability in histidine peptides by β-alanine
- Source :
- Journal of Inorganic Biochemistry. 119:85-89
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The cooperativity of formation of 5-membered and 6-membered chelate rings is the driving force for specificity and selectivity in Cu(II) peptidic complexes. α-Amino acids enable the formation of 5-membered rings, while a 6-membered ring is provided by the coordination of the His side chain imidazole. Introduction of β-alanine is another way of creating a 6-membered ring in the Cu(II) complex. The potentiometric and spectroscopic (UV–vis and CD) study of Cu(II) complexation by a series of four peptides, AAH-am, ABH-am, BAH-am, and BBH-am (where B stands for β-alanine, and -am for C-terminal amide) revealed a very strong effect of the sizes of individual rings, with the order of complex stability AAH-am (5,5,6) > BAH-am (6,5,6) > ABH-am (5,6,6) ≫ BBH-am (6,6,6). The stabilities of ABH-am and BAH-am complexes are intermediate between those of strong His-3 peptides but these complexes are still able to saturate the coordination sphere of the Cu(II) ion at neutral pH. This fact opens up new possibilities in engineering specific peptide-based chelates.
- Subjects :
- Coordination sphere
Cations, Divalent
Protein Stability
Chemistry
Stereochemistry
Circular Dichroism
Cooperativity
Biochemistry
Inorganic Chemistry
chemistry.chemical_compound
Coordination Complexes
Stability constants of complexes
Amide
Potentiometry
beta-Alanine
Side chain
Imidazole
Histidine
Selectivity
Oligopeptides
Copper
Subjects
Details
- ISSN :
- 01620134
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Inorganic Biochemistry
- Accession number :
- edsair.doi.dedup.....1987b999abc99269380ab326e186252d