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Chlamydia trachomatis impairs host base excision repair by downregulating polymerase β
Chlamydia trachomatis impairs host base excision repair by downregulating polymerase β
- Source :
- Cellular microbiology. 21(4)
- Publication Year :
- 2018
-
Abstract
- Chlamydia trachomatis infections have been associated with ovarian cancer by several epidemiological studies. Here, we show that C. trachomatis-infected primary human ovarian epithelial cells display elevated oxidative DNA damage. Base excision repair, an important cellular mechanism to repair oxidative DNA lesions, was impaired in infected primary ovarian and in several other types of cells. Polymerase β was downregulated in infected cells associated with upregulation of microRNA-499a (miR-499a). Stabilising polymerase β by inhibiting miR-499a significantly improved repair. Moreover, downregulation of tumour suppressor p53 also resulted in attenuated repair in these cells. Thus, our data show that downregulation of polymerase β by direct inhibition through miR-499a and downregulation of p53 debilitate the host-cell base excision repair during C. trachomatis infection.
- Subjects :
- DNA Repair
Immunology
Immunoblotting
Down-Regulation
Chlamydia trachomatis
Oxidative phosphorylation
Biology
medicine.disease_cause
Real-Time Polymerase Chain Reaction
Microbiology
law.invention
03 medical and health sciences
chemistry.chemical_compound
Downregulation and upregulation
law
Virology
medicine
Humans
030304 developmental biology
0303 health sciences
030306 microbiology
Ovary
Epithelial Cells
Base excision repair
Chlamydia Infections
medicine.disease
Real-time polymerase chain reaction
chemistry
Cancer research
Suppressor
Female
Ovarian cancer
DNA
DNA Damage
Subjects
Details
- ISSN :
- 14625822
- Volume :
- 21
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cellular microbiology
- Accession number :
- edsair.doi.dedup.....199d56ca6cf80c5d681d9d428a86e7fc