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Intestinal toxicity of the new type A trichothecenes, NX and 3ANX
- Source :
- Chemosphere, Chemosphere, Elsevier, 2022, 288 (1), ⟨10.1016/j.chemosphere.2021.132415⟩
- Publication Year :
- 2022
- Publisher :
- HAL CCSD, 2022.
-
Abstract
- International audience; NX and its acetylated form 3ANX are two new type A trichothecenes produced by Fusarium graminearum whose toxicity is poorly documented. The aim of this study was to obtain a general view of the intestinal toxicity of these toxins. Deoxynivalenol (DON), which differs from NX by the keto group at C8, served as a benchmark. The viability of human intestinal Caco-2 cells decreased after 24 h of exposure to 3 μM NX (−21.4%), 3 μM DON (−20.2%) or 10 μM 3ANX (−17.4%). Histological observations of porcine jejunal explants exposed for 4 h to 10 μM of the different toxins showed interstitial edema and cellular debris. Explants exposed to NX also displayed cell vacuolization, a broken epithelial barrier and high loss of villi. Whole transcriptome profiling revealed that NX, DON and 3ANX modulated 369, 146 and 55 genes, respectively. Functional analyses indicated that the three toxins regulate the same gene networks and signaling pathways mainly; cell proliferation, differentiation, apoptosis and growth, and particularly immune and pro-inflammatory responses. Greater transcriptional impacts were observed with NX than with DON. In conclusion, our data revealed that the three toxins have similar impacts on the intestine but of different magnitude: NX > DON ≫ 3ANX. NX and 3ANX should consequently be included in overall risk analysis linked to the presence of trichothecenes in our diet.
- Subjects :
- Fusarium
Environmental Engineering
Swine
Health, Toxicology and Mutagenesis
Cell
Explant
Trichothecenes, Type A
Transcriptome
03 medical and health sciences
Immune system
medicine
Environmental Chemistry
Animals
Humans
Gut
Intestinal Mucosa
030304 developmental biology
0303 health sciences
biology
Cell growth
Chemistry
Gene Expression Profiling
030302 biochemistry & molecular biology
Public Health, Environmental and Occupational Health
General Medicine
General Chemistry
biology.organism_classification
Pollution
Molecular biology
Deoxynivalenol
3. Good health
Fusarium graminearum
medicine.anatomical_structure
Vacuolization
Apoptosis
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Toxicity
Caco-2 Cells
Trichothecenes
Subjects
Details
- Language :
- English
- ISSN :
- 00456535
- Database :
- OpenAIRE
- Journal :
- Chemosphere, Chemosphere, Elsevier, 2022, 288 (1), ⟨10.1016/j.chemosphere.2021.132415⟩
- Accession number :
- edsair.doi.dedup.....199f1fea2a3dd81ce8686131f3b8ebd6