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Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles
- Source :
- Molecules, Molecules, Vol 25, Iss 4345, p 4345 (2020), Volume 25, Issue 18
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done.
- Subjects :
- Antioxidant
Cell Survival
Swine
medicine.medical_treatment
Size-exclusion chromatography
polysaccharides
Pharmaceutical Science
truffle
Oxidative phosphorylation
Polysaccharide
Antioxidants
Article
Analytical Chemistry
lcsh:QD241-441
lcsh:Organic chemistry
Ascomycota
Drug Discovery
medicine
Animals
structure
Food science
Viability assay
Physical and Theoretical Chemistry
Aroma
Tuber panzhihuanense
Plant Proteins
chemistry.chemical_classification
Truffle
biology
Tuber pseudoexcavatum
Organic Chemistry
Epithelial Cells
Fungal Polysaccharides
biology.organism_classification
chemistry
Cytoprotection
Chemistry (miscellaneous)
Yield (chemistry)
Molecular Medicine
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....19c8fb0a194dfebadd62a2da7297e097