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Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis
- Source :
- Methods Mol Biol, Methods in Molecular Biology ISBN: 9781071619230
- Publication Year :
- 2022
-
Abstract
- Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes.
Details
- Language :
- English
- ISBN :
- 978-1-07-161923-0
- ISBNs :
- 9781071619230
- Database :
- OpenAIRE
- Journal :
- Methods Mol Biol, Methods in Molecular Biology ISBN: 9781071619230
- Accession number :
- edsair.doi.dedup.....19d9bae40dc5f29c4dc1d33488933c27