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Optimal Use of MRSASelect and PCR to Maximize Sensitivity and Specificity of MRSA Detection
- Source :
- Current Microbiology. 66:61-63
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher than that of 48 h (91.4 vs. 60 %), extending the incubation time to 48 h, along with PCR confirmation, increased the total number of true positive samples by 27.8 %. These results provide a cost effective method for sensitive and specific detection of MRSA.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Bacteriological Techniques
Chromogenic
Specific detection
General Medicine
Staphylococcal Infections
Biology
medicine.disease_cause
Sensitivity and Specificity
Applied Microbiology and Biotechnology
Microbiology
Incubation period
Molecular Diagnostic Techniques
Staphylococcus aureus
medicine
Humans
Multiplex
Incubation
Subjects
Details
- ISSN :
- 14320991 and 03438651
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Current Microbiology
- Accession number :
- edsair.doi.dedup.....19dd25e2d8bd7a62f0aee7e71cc38b84