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Loss of H3K27me3 in meningiomas
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Neuro Oncol, Neuro-Oncology 23(8), 1282-1291 (2021). doi:10.1093/neuonc/noab036
- Publication Year :
- 2021
-
Abstract
- Background There is a critical need for objective and reliable biomarkers of outcome in meningiomas beyond WHO classification. Loss of H3K27me3 has been reported as a prognostically unfavorable alteration in meningiomas. We sought to independently evaluate the reproducibility and prognostic value of H3K27me3 loss by immunohistochemistry (IHC) in a multicenter study. Methods IHC staining for H3K27me3 and analyses of whole slides from 181 meningiomas across three centers was performed. Staining was analyzed by dichotomization into loss and retained immunoreactivity, and using a 3-tiered scoring system in 151 cases with clear staining. Associations of grouping with outcome were performed using Kaplan-Meier survival estimates. Results A total of 21 of 151 tumors (13.9%) demonstrated complete loss of H3K27me3 staining in tumor with retained endothelial staining. Overall, loss of H3K27me3 portended a worse outcome with shorter times to recurrence in our cohort, particularly for WHO grade 2 tumors which were enriched in our study. There were no differences in recurrence-free survival (RFS) for WHO grade 3 patients with retained vs loss of H3K27me3. Scoring by a 3-tiered system did not add further insights into the prognostic value of this H3K27me3 loss. Overall, loss of H3K27me3 was not independently associated with RFS after controlling for WHO grade, extent of resection, sex, age, and recurrence status of tumor on multivariable Cox regression analysis. Conclusions Loss of H3K27me3 identifies a subset of WHO grade 2 and possibly WHO grade 1 meningiomas with increased recurrence risk. Pooled analyses of a larger cohort of samples with standardized reporting of clinical definitions and staining patterns are warranted.
- Subjects :
- Oncology
Cancer Research
medicine.medical_specialty
macromolecular substances
Meningioma
Histones
03 medical and health sciences
0302 clinical medicine
Internal medicine
Meningeal Neoplasms
Medicine
Humans
Meningeal Neoplasm
ddc:610
030304 developmental biology
0303 health sciences
REPRODUTIBILIDADE DE RESULTADOS
business.industry
Proportional hazards model
Editorials
Cancer
Reproducibility of Results
medicine.disease
Prognosis
Staining
030220 oncology & carcinogenesis
Cohort
Immunohistochemistry
Neurology (clinical)
Neoplasm Recurrence, Local
business
Who classification
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Neuro Oncol, Neuro-Oncology 23(8), 1282-1291 (2021). doi:10.1093/neuonc/noab036
- Accession number :
- edsair.doi.dedup.....19df5fe9240c9e8d2ccea4ec14691828