Modelling filovirus maintenance in nature by experimental transmission of Marburg virus between Egyptian rousette bats

The Egyptian rousette bat (ERB) is a natural reservoir host for Marburg virus (MARV); however, the mechanisms by which MARV is transmitted bat-to-bat and to other animals are unclear. Here we co-house MARV-inoculated donor ERBs with naive contact ERBs. MARV shedding is detected in oral, rectal and urine specimens from inoculated bats from 5–19 days post infection. Simultaneously, MARV is detected in oral specimens from contact bats, indicating oral exposure to the virus. In the late study phase, we provide evidence that MARV can be horizontally transmitted from inoculated to contact ERBs by finding MARV RNA in blood and oral specimens from contact bats, followed by MARV IgG antibodies in these same bats. This study demonstrates that MARV can be horizontally transmitted from inoculated to contact ERBs, thereby providing a model for filovirus maintenance in its natural reservoir host and a potential mechanism for virus spillover to other animals.
Bats are natural hosts for Marburg virus (MARV), but the mechanism of bat-to-bat transmission is unclear. Here, Schuh et al. monitor MARV infection in a cohort of 38 bats over nine months, find ‘supershedders' and show that MARV can horizontally transmit between bats.