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Evaluation Of Nitrate Reductase Assay For Early Detection Of Multi And Extensively Drug Resistance Tuberculosis In Our Setup
- Source :
- Journal of College of Physicians And Surgeons Pakistan. 28:22-25
- Publication Year :
- 2018
- Publisher :
- College of Physicians and Surgeons Pakistan, 2018.
-
Abstract
- Objective To evaluate the performance of nitrate reductase assay on smear positive pulmonary specimens for detection of multi and extensively drug resistant tuberculosis simultaneously. Study design Cross-sectional analytical study. Place and duration of study Microbiology Department, Armed Forces Institute of Pathology, Rawalpindi from June to December 2016. Methodology Smear positive pulmonary samples were processed both by nitrate reductase method on Lowenstein Jenson medium and also inoculated on gold standard Bactec MGIT 960 TB system. All the specimens were first digested and decontaminated according to standard protocol before inoculation. Results Out of total 76 samples, three did not give color and, therefore, were excluded from the final data analysis. Among the remaining 73 samples, mycobacterial index was: 28 specimens were having 1+ (1-9 bacilli/100 fields), 26 samples were 2+ (1-9 bacilli/ field), and 19 samples were having 3+ index (>9 bacilli/field). The respective sensitivity and specificity were 84% and 100% for isoniazid (INH); 82% and 100% for rifampin (RIF); 67% and 100% for amikacin (AK); and both 100% for ofloxacin (OFX). Overall agreement in case of INH, RIF, AK, and OFX was 94.5%, 97.2%, 98.6% and100%, respectively. Overall average agreement was 97.5%. Conclusion Nitrate reductase assay is a reliable, low cost and accurate method that can be used for early for diagnosis of multi and extensively drug resistant tuberculosis.
- Subjects :
- 0301 basic medicine
Veterinary medicine
Tuberculosis
Extensively Drug-Resistant Tuberculosis
030231 tropical medicine
Antitubercular Agents
Microbial Sensitivity Tests
Drug resistance
Nitrate reductase
Nitrate Reductase
Sensitivity and Specificity
Mycobacterium tuberculosis
03 medical and health sciences
0302 clinical medicine
Predictive Value of Tests
Drug Resistance, Multiple, Bacterial
Tuberculosis, Multidrug-Resistant
Isoniazid
medicine
Humans
Amikacin
biology
business.industry
Sputum
Extensively drug-resistant tuberculosis
General Medicine
bacterial infections and mycoses
biology.organism_classification
medicine.disease
Cross-Sectional Studies
Early Diagnosis
030104 developmental biology
Ofloxacin
Rifampin
business
medicine.drug
Subjects
Details
- ISSN :
- 16817168 and 1022386X
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of College of Physicians And Surgeons Pakistan
- Accession number :
- edsair.doi.dedup.....1a05739c70a9b97758e691db4d4b8f4f