Pyrosequencing-based barcodes for a dye-free multiplex bioassay

Authors :: Haiping Wu
Bingjie Zou
Hideki Kambara
Jinheng Li
Zhiyao Chen
Xi-qun Liu
Xiaodan Zhang
Tomoharu Kajiyama
Xiaoying Fu
Guohua Zhou
Qinxin Song
Source :: Chemical communications (Cambridge, England). 48(18)
Publication Year :: 2012
Abstract: A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

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