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Revealing the Dynamic Mechanism by Which Transferrin Promotes the Cellular Uptake of HAIYPRH Peptide-Conjugated Nanostructures by Force Tracing
- Source :
- Molecular Pharmaceutics. 18:1480-1485
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- The HAIYPRH (T7) peptide has been widely used as a ligand for constructing tumor-targeted nanodrug delivery systems since it can target the transferrin receptor (TfR) and then enter cells easily with the help of transferrin (Tf). However, the dynamic mechanism by which transferrin promotes the entry of T7-conjugated nanostructures into cells remains unclear. Herein, a force tracing technique based on atomic force microscopy (AFM) was used to track the ultrafast dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering a cell at the single-particle level in real time. Tf helped decrease the endocytosis force and increase the endocytosis speed of AuNP-T7 in A549 cells. However, Tf only increased the endocytosis speed of AuNP-T7 in HeLa cells. In contrast, in Vero cells without TfR overexpression, Tf decreased the endocytosis speed. This report provides important insights for redesigning and developing T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
- Subjects :
- Cell
Metal Nanoparticles
Pharmaceutical Science
Transferrin receptor
02 engineering and technology
Endocytosis
030226 pharmacology & pharmacy
HeLa
03 medical and health sciences
Drug Delivery Systems
0302 clinical medicine
Cell Line, Tumor
Chlorocebus aethiops
Receptors, Transferrin
Drug Discovery
medicine
Animals
Humans
Vero Cells
chemistry.chemical_classification
A549 cell
biology
Transferrin
Biological Transport
021001 nanoscience & nanotechnology
Ligand (biochemistry)
biology.organism_classification
Nanostructures
medicine.anatomical_structure
chemistry
A549 Cells
Biophysics
Vero cell
Molecular Medicine
Gold
Peptides
0210 nano-technology
HeLa Cells
Subjects
Details
- ISSN :
- 15438392 and 15438384
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Molecular Pharmaceutics
- Accession number :
- edsair.doi.dedup.....1a11d4c0a10a0a45372120177c9feb34