Additional file 1 of Plant sex affects plant-microbiome assemblies of dioecious Populus cathayana trees under different soil nitrogen conditions

Additional file 1: Supplementary methods. Table S1. Bacterial and fungal co-occurrence network characteristics in each niche. Table S2. The physiological traits and microbial functions of rhizosphere soil affected by sex and nitrogen treatment. Table S3. The growth characteristics of P. cathayana males and females under different N treatments. Figure S1. The composition and relative abundance of bacterial (a) and fungal (b) communities in the rhizosphere soil, roots, old leaves and young leaves of P. cathayana males and females in different N treatments. Figure S2. The Shannon diversity of bacterial and fungal communities in different N treatments. From left to right: rhizosphere soil, roots, old leaves and young leaves. Figure S3. The volcano plot illustrating the enrichment and depletion patterns of bacterial and fungal microbiomes in each niche (male vs female). Figure S4. Different niche effects on the networks of bacterial (a) and fungal (b) communities. Figure S5. Phylogenetic tree, taxonomic composition, and distribution patterns of dominant fungal taxa living in roots, old leaves and young leaves. (a) Dominant fungal taxa in P. cathayana females and males, respectively. (b) The distribution pattern of dominant fungal taxa in P. cathayana females. (c) The number of dominant fungal taxa, the shared and specific bacterial OTUs in P. cathayana females and males. (d) The distribution pattern of dominant fungal taxa in P. cathayana males. Figure S6. Heatmaps of the expression profiles (a), differential expression analysis (DEGs) (b) and functional-enrichment analysis (KEGG) (c). MC, young male leaves in control-nitrogen treatment; MN, young male leaves in limited-nitrogen treatment; FC, young female leaves in control-nitrogen treatment; FN, young female leaves in limited-nitrogen treatment.