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Cross-resistance of isoniazid, para-aminosalicylic acid and pasiniazid against isoniazid-resistant Mycobacterium tuberculosis isolates in China
- Source :
- Journal of Global Antimicrobial Resistance, Vol 20, Iss, Pp 275-281 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Objectives Pasiniazid is a chemical complex of isoniazid (INH) and para-aminosalicylic acid (PAS). The aim of this study was to explore the cross-resistance of INH, PAS and pasiniazid against INH-resistant Mycobacterium tuberculosis isolates in China. Methods A Microplate alamarBlue® Assay was performed to determine the minimum inhibitory concentrations (MICs) of INH, PAS and pasiniazid against 109 INH-resistant M. tuberculosis isolates. A statistical analysis of the relationship between different genotypes, gene mutations, and INH, PAS or pasiniazid susceptibility was then performed. Results Among the 109 INH-resistant isolates, 13 (11.9%) and 21 (19.3%) showed resistance to PAS and pasiniazid, respectively. Among the 13 PAS-resistant M. tuberculosis isolates, 11 remained susceptible to pasiniazid. Of 63 INH-resistant isolates harbouring mutations in katG, the inhA promoter or the oxyR–ahpC intergenic region, 52 remained susceptible to pasiniazid. Moreover, 11 of 13 pasiniazid-resistant isolates carried mutations in katG, the inhA promoter or the oxyR–ahpC intergenic region. Conclusion Taken together, these results demonstrate that PAS resistance and mutations in thekatG gene, inhA promoter or oxyR–ahpC intergenic region in INH-resistant M. tuberculosis have little effect on pasiniazid susceptibility.
- Subjects :
- 0301 basic medicine
Antitubercular Agents
Gene mutation
0302 clinical medicine
Intergenic region
Drug Resistance, Multiple, Bacterial
Genotype
Immunology and Allergy
heterocyclic compounds
030212 general & internal medicine
Promoter Regions, Genetic
INHA
Isoniazid
respiratory system
Catalase
Aminosalicylic Acid
QR1-502
DNA, Intergenic
Oxidoreductases
medicine.drug
Adult
Microbiology (medical)
China
Tuberculosis
030106 microbiology
Immunology
Microbial Sensitivity Tests
Biology
Microbiology
Mycobacterium tuberculosis
03 medical and health sciences
Bacterial Proteins
medicine
Humans
Cross-resistance
Tuberculosis, Pulmonary
Pasiniazid
Peroxiredoxins
biochemical phenomena, metabolism, and nutrition
medicine.disease
biology.organism_classification
bacterial infections and mycoses
Para-aminosalicylic acid
respiratory tract diseases
Repressor Proteins
Aminosalicylic Acids
Mutation
Subjects
Details
- Language :
- English
- ISSN :
- 22137165
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Global Antimicrobial Resistance
- Accession number :
- edsair.doi.dedup.....1a3b78d91c19299ff634bed7eb264fcd