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Aberrant histone acetylation and methylation levels in woman with endometriosis
- Source :
- Archives of gynecology and obstetrics. 287(3)
- Publication Year :
- 2012
-
Abstract
- To investigate the alterations in histone modifications in woman with endometriosis.Global histone H3/H4 acetylation and H3K4/H3K9 methylation in eutopic and ectopic endometrium from 15 endometriosis patients were assayed using the EpiQuik global histone H3/H4 acetylation and H3K4/H3K9 methylation assay kits. Quantitative real-time reverse transcriptase-polymerase chain reaction was applied to measure mRNA levels of 12 members of histone-related chromatin modifier genes.Histone H4 hypoacetylation was detected both in eutopic and ectopic endometrium. There were no difference between patients with endometriosis and controls on global levels of H3 acetylation. Furthermore, global histone H3K4 hypomethylation and H3K9 hypomethylation were detected both in ectopic and eutopic endometrium (p0.001), and in ectopic endometrium (p0.001), respectively. SIRT1 mRNA level was significantly decreased in eutopic endometrium, while mRNA levels of HDAC1, SUV39H1, SUV39H2 and G9a were significantly downregulated in ectopic endometrium. HDAC2 mRNA level was significantly increased in eutopic endometrium. PCAF mRNA level was significantly increased in ectopic endometrium.Aberrant histone modification may play an important role in the pathogenesis of endometriosis.
- Subjects :
- Adult
genetic structures
Endometriosis
Endometrium
Methylation
Histone Deacetylases
Epigenesis, Genetic
Histones
Young Adult
Medicine
Humans
Cancer epigenetics
RNA, Messenger
Epigenesis
Histone Acetyltransferases
biology
business.industry
Obstetrics and Gynecology
Acetylation
General Medicine
Histone-Lysine N-Methyltransferase
medicine.disease
Histone
medicine.anatomical_structure
Case-Control Studies
biology.protein
Cancer research
Female
business
Protein Processing, Post-Translational
Subjects
Details
- ISSN :
- 14320711
- Volume :
- 287
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Archives of gynecology and obstetrics
- Accession number :
- edsair.doi.dedup.....1a749d94fb39bc90b195d01eaa37ab6a