Back to Search
Start Over
A technique for the measurement of orthophosphate in human erythrocytes, and some studies of its determinants
- Source :
- Clinical science (London, England : 1979). 69(4)
- Publication Year :
- 1985
-
Abstract
- 1. A technique is described for the determination of orthophosphate (Pi) in human erythrocytes. The advantages of the technique are that it uses whole blood rather than separated erythrocytes, that it avoids major hydrolysis of organic phosphates, that it takes account of incomplete recovery of Pi and that it minimizes the effects of chilling the cells. 2. In chilled samples from 46 patients in an intensive care unit, the cellular concentration of Pi was proportional to that in plasma. 3. Blood samples from nine normal subjects were incubated at 37°C. The cellular Pi was 0.79 mmol/litre of cells using an external standardization and 0.67 using an internal standardization. When the same cell samples were chilled on ice for 30 min, the internally standardized value decreased further to 0.57 mmol/litre of cells. These results suggest that differences in recovery, and the extent of chilling, contribute to the variability in the previously reported values for erythrocyte Pi. 4. If Pi, like chloride, had distributed passively between cells and plasma in these samples, the cell to plasma molar concentration ratio for Pi should have been 0.29, compared with the measured value of 0.64. This difference suggests that some factor, in addition to passive diffusion, determined the distribution of Pi.
- Subjects :
- Chromatography
Molar concentration
Erythrocytes
Phosphates/*blood
Liter
General Medicine
Phosphate
Chloride
Phosphates
Blood cell
Cold Temperature
Red blood cell
chemistry.chemical_compound
medicine.anatomical_structure
chemistry
Hematocrit
Mole
medicine
Erythrocytes/*metabolism
Methods
Humans
medicine.drug
Whole blood
Subjects
Details
- ISSN :
- 01435221
- Volume :
- 69
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Clinical science (London, England : 1979)
- Accession number :
- edsair.doi.dedup.....1aa291d64b25205b1aa009504e4b79ae