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HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 22, International Journal of Molecular Sciences, Vol 22, Iss 12241, p 12241 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Mesenchymal stem cells (MSCs) can improve chronic wound healing<br />however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through the growth factors and cytokines secreted by these cells, referred to as the MSC secretome. To overcome difficulties related to the translation of cell therapy into clinical use such as efficacy, safety and cost, we propose a hydrogel loaded with a secretome from the recently established human adipose tissue mesenchymal stem cell line (HATMSC2) as a potential treatment for chronic wounds. Biocompatibility and biological activity of hydrogel-released HATMSC2 supernatant were investigated in vitro by assessing the proliferation and metabolic activity of human fibroblast, endothelial cells and keratinocytes. Hydrogel degradation was measured using hydroxyproline assay while protein released from the hydrogel was assessed by interleukin-8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) ELISAs. Pro-angiogenic activity of the developed treatment was assessed by tube formation assay while the presence of pro-angiogenic miRNAs in the HATMSC2 supernatant was investigated using real-time RT-PCR. The results demonstrated that the therapeutic effect of the HATMSC2-produced factors is maintained following incorporation into collagen hydrogel as confirmed by increased proliferation of skin-origin cells and improved angiogenic properties of endothelial cells. In addition, HATMSC2 supernatant revealed antimicrobial activity, and which therefore, in combination with the hydrogel has a potential to be used as advanced wound-healing dressing.
- Subjects :
- Chronic wound
Keratinocytes
Adipose tissue
adipose tissue-derived mesenchymal stem cells
Cell therapy
Anti-Infective Agents
Macrophage
Biology (General)
chronic wound
Spectroscopy
Secretome
Skin
MSC secretome
Tube formation
integumentary system
Chemistry
food and beverages
Hydrogels
General Medicine
Computer Science Applications
medicine.anatomical_structure
Adipose Tissue
medicine.symptom
QH301-705.5
Cell Survival
Catalysis
Article
Cell Line
Inorganic Chemistry
medicine
Humans
collagen hydrogel
Physical and Theoretical Chemistry
Fibroblast
QD1-999
Molecular Biology
Cell Proliferation
Bacteria
Organic Chemistry
Mesenchymal stem cell
fungi
Endothelial Cells
Mesenchymal Stem Cells
Fibroblasts
In vitro
mesenchymal stem cell secretome
MicroRNAs
Culture Media, Conditioned
Cancer research
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....1adf0c54fcde5950b127c661ff570bdb
- Full Text :
- https://doi.org/10.3390/ijms222212241