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Effect of cold aerobic perfusion on nonparenchymal cell viability of rat livers
- Source :
- Transplant International. 7
- Publisher :
- Frontiers Media SA
-
Abstract
- We investigated the effect of oxygen supply on hepatic cellular viability during cold perfusion storage of rat livers. A perfluoro-N-methyldecahydroisoquinoline (FMIQ) emulsion is used as an oxygen carrier. The composition of the perfusate containing 20 w/v% FMIQ is essentially the same as the University of Wisconsin (UW) solution except for the exclusion of hydroxyethyl starch. Rat livers were perfused at 4 degrees C for up to 24 h with either UW solution (group I, oxygenated; group II, unoxygenated) or FMIQ solution (group III, oxygenated; group IV, unoxygenated). After perfusion storage, the livers were reperfused with warm (37 degrees C) oxygenated or cold (4 degrees C) unoxygenated Krebs-Henseleit bicarbonate buffer, and nuclear trypan blue uptake was measured as the index of cell death. With warm oxygenated reperfusion, there remained less than 2% noviable parenchymal cells up to 24 h, regardless of perfusate or oxygenation. In UW-perfused livers, the proportion of nonviable nonprenchymal cells (NPC) increased progressively regardless of oxygenation, the values in groups I and II in the periportal field at 24 h being 39.9 +/- 4.7% (mean +/- SD) and 36.5 +/- 4.2%, respectively. By contrast, in FMIQ-perfused livers, dye uptake by NPC was significantly reduced with oxygenation (16.9 +/- 5.7% and 39.4% +/- 9.1% at 24 h in groups III and IV; P < 0.001). With cold unoxygenated reperfusion, livers in groups I, II, and IV showed a significant decrease of nonviable NPC, while those in group III showed no significant changes. These data indicate that oxygen supply during perfusion storage of the liver may ameliorate lethal injury to NPC precipitated during reperfusion.
- Subjects :
- Male
Adenosine
Cell Survival
Bicarbonate
Allopurinol
Organ Preservation Solutions
Hydroxyethyl starch
Andrology
Rats, Sprague-Dawley
chemistry.chemical_compound
Raffinose
Blood Substitutes
Medicine
Animals
Insulin
Viaspan
Viability assay
Liver preservation
Fluorocarbons
Transplantation
business.industry
Oxygenation
Trypan Blue
Isoquinolines
Glutathione
Aerobiosis
Rats
Perfusion
chemistry
Biochemistry
Liver
Reperfusion
Trypan blue
business
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 09340874 and 14322277
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Transplant International
- Accession number :
- edsair.doi.dedup.....1b0d78fd379252197ea15a5e9454a772
- Full Text :
- https://doi.org/10.1111/j.1432-2277.1994.tb01341.x